Membrane proteins
Membrane Proteins - Engineering, Purification and Crystallization, a volume of Methods In Enzymology, encompasses chapters from the leading experts in the area of membrane protein biology. The chapters provide a brief overview of the topics covered and also outline step-by-step protocol for the interested audience. Illustrations and case example images are included wherever appropriate to help the readers understand the schematics and general experimental outlines. Volume of Methods In Enzymolo…
Read More ↓Title Statements
- Full title :Membrane proteins. / edited by Arun K. Shukla.
Summary
Membrane Proteins - Engineering, Purification and Crystallization, a volume of Methods In Enzymology, encompasses chapters from the leading experts in the area of membrane protein biology. The chapters provide a brief overview of the topics covered and also outline step-by-step protocol for the interested audience. Illustrations and case example images are included wherever appropriate to help the readers understand the schematics and general experimental outlines. Volume of Methods In EnzymologyContains a collection of a diverse array of topics in the area of membrane protein biology rangin.
Read More ↓Edition Statements
- Publication Date:©2015
Publication Statements
- Publisher(s):Academic Press
- Place of Publication:Waltham, MA
- Language:eng
- Publication Date:©2015
The Physical Object
- Description:1 online resource (xx, 624 pages) : : illustrations (chiefly color)
Edition Identifiers
- ISBN:9780128021934, 0128021934
- oclc:ocn908335688
- LC Classification:QP552.M44
- Dewey Decimal:572/.696
Table of Contents
Front Cover; Membrane Proteins-Engineering, Purification and Crystallization; Copyright; Contents; Contributors; Preface; Section I: Membrane Protein Engineering, Solubilization and Purification; Chapter One: Multicolor Fluorescence-Based Screening Toward Structural Analysis of Multiprotein Membrane Complexes; 1. Introduction; 1.1. Screening approaches for membrane proteins and membrane protein complexes; 1.2. Multicolor fluorescence-based screening of multiprotein membrane complexes.
1.3. The major histocompatibility complex class I peptide-loading complex: A challenge for structural biology of membrane ... 2. Production of Fluorescently Labeled TAP Complexes in P. pastoris; 2.1. Construct design; 2.2. Transformation; 2.3. Colony screening; 2.4. Large-scale expression; 2.5. Orthogonal purification; 3. Production of Fluorescently Labeled TAP Complexes in Mammalian Cells; 3.1. Construct design; 3.2. Transfection; 3.3. Orthogonal purification; 4. Multicolor Fluorescence-Based Screening Approaches; 4.1. MC-FSEC analysis; 4.2. In-gel fluorescence and immunoblotting.
4.3. Monitoring peptide binding to TAP4.4. Measurement of MHC I surface presentation by flow cytometry; 5. Summary; Ac…
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