Methods in cell biology

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The goal of this book is to collect methods and protocols for studying cilia in a wide range of different cell types, so that researchers from many fields of biology can start exploring the role of cilia in their own system. <li>Chapters are written by experts in the field</li><li>Cutting-edge material</li>

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Title Statements

Full title :Methods in cell biology. / edited by Renata Basto, Wallace F. Marshall.

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Edition Statements

Publication Date:2015.

Publication Statements

Publisher(s):Academic Press, Elsevier
Place of Publication:Amsterdam
Language:eng
Publication Date:2015.

The Physical Object

Description:1 online resource

Edition Identifiers

ISBN:9780128026403, 0128026405
oclc:ocn905854011
LC Classification:QH581.2
Dewey Decimal:571.6

Front Cover; Methods in Cell Biology; Series Editors; Methods in Cell BiologyMethods in Cilia & FlagellaVolume 127Edited byRenata BastoInstitut Curie, FranceWallace F. MarshallD ... ; Contents; Contributors; Preface; 1. Imaging centrosomes and cilia in the mouse kidney; Introduction; 1. Methods; 1.1 Isolation of Kidneys from Adult Mouse; 1.2 Isolation of Kidneys from Mouse Embryo; 1.3 Fixation and Preparation of Slides; 1.4 Antibody Staining of Fixed Samples; 1.4.1 Antigen retrieval; 1.4.2 Antibody staining; 1.4.3 Fluorescence microscopy; 1.5 Ex vivo Culture of Embryonic Kidney; Conclusion.

2.2.1 Materials2.2.2 Method; 2.2.3 Analysis; 3. Localization of PCP Signaling Pathway Proteins in Multiciliated Airway Epithelial Cells; 3.1 Observing Vang-Like 1 and Frizzled 6 Localization in the In vivo Multiciliated Airway Epithelium; 3.1.1 Materials; 3.1.2 Method; 3.1.3 Analysis; 3.2 Observing Vang-Like 1 and Frizzled 6 Localization in In vitro Differentiated Multiciliated Airway Epithelial Cells; 3.2.1 Materials; 3.2.2 Method; 3.2.3 Analysis; Conclusions; Acknowledgments; References; 4. Imaging and analyzing primary cilia in cardiac cells; Introduction.

1. Imaging Cilia In situ, in Fixe…

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