Methods in enzymology

Methods in enzymology

by Handel, Tracy M.

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Title Statements

  • Full title :Methods in enzymology. / edited by Tracy M. Handel.

Edition Statements

  • Publication Date:©2016
  • Edition:First edition

Publication Statements

  • Publisher(s):Academic Press
  • Place of Publication:Amsterdam, [Netherlands]
  • Language:eng
  • Publication Date:©2016

Notes

Description based upon print version of record.

Chapter Four: Methods for the Recognition of GAG-Bound Chemokines.

The Physical Object

  • Description:1 online resource (676 p.).

Edition Identifiers

  • ISBN:0128021950, 9780128021958, 0128021713, 9780128021712
  • oclc:on1316618401
  • LC Classification:QR185.8.C45
  • Dewey Decimal:616.079

Table of Contents

Front Cover; Chemokines; Copyright; Contents; Contributors; Preface; Chapter One: Chemokine Detection Using Receptors Immobilized on an SPR Sensor Surface; 1. Surface Plasmon Resonance; 2. Chemokine Receptors: Members of the GPCR Family; 3. Chemokine Receptor Immobilization on the Sensor Chip; 4. Viral Particles as Chemokine Receptors Carriers in SPR; 4.1. Materials; 4.2. Method I: Generation of Retroviral Particles; 4.3. Method II: Generation of LVPs; 4.4. Method III: Titration of Viral Proteins; 4.5. Method IV: Determination of Chemokine Receptor Levels on the VP

4.6. Method V: Quantitation of Receptor Number on VPs4.7. Method VI: Attachment of VPs to Biosensor Surfaces; 5. SPR-Based Applications for Chemokine Receptors; 6. Conclusions; Acknowledgments; References; Chapter Two: Study of Chemotaxis and Cell-Cell Interactions in Cancer with Microfluidic Devices; 1. Introduction; 2. Methods; 2.1. Chemotaxis in Microfluidic Chemotaxis Chamber; 2.1.1. Making Microfluidic Chemotaxis Chamber; 2.1.2. Chemotaxis Assay for Differentiated HL-60 Cells; 2.2. Gradient Switching in Microfluidic Device; 2.2.1. Making a Gradient Switching Microfluidic Device

2.2.2. Chemotaxis Assay for Differe…

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Subjects

Chemokines Chemokines -- Physiological effect

Writers

Handel, Tracy M.

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