NADPH Oxidases: Methods and Protocols
by Knaus, Ulla G. Leto, Thomas L.
This detailed volume explores the NADPH oxidase family of enzymes in human physiology and genetic disease, in which early discoveries represent prime examples of the finest translational "from bed to bench and back" studies. Methods are included for testing assembly and function of multicomponent oxidase complexes and for analyzing reactive oxygen species (ROS) generation in different systems by various means, while addressing pitfalls of ROS probes currently being used, as well as protocols on…
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- Full title :NADPH Oxidases : Methods and Protocols / edited by Ulla G. Knaus, Thomas L. Leto.
Summary
This detailed volume explores the NADPH oxidase family of enzymes in human physiology and genetic disease, in which early discoveries represent prime examples of the finest translational "from bed to bench and back" studies. Methods are included for testing assembly and function of multicomponent oxidase complexes and for analyzing reactive oxygen species (ROS) generation in different systems by various means, while addressing pitfalls of ROS probes currently being used, as well as protocols on NADPH oxidase regulation and their function in cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, NADPH Oxidases: Methods and Protocols will aid researchers working with the NOX/DUOX family in continuing and expanding upon their vital research.
Read More ↓Edition Statements
- Publication Date:2019.
Publication Statements
- Publisher(s):Imprint: Humana, Springer New York
- Place of Publication:New York, NY
- Language:eng
- Publication Date:2019.
The Physical Object
- Description:1 online resource (XV, 718 p. 155 illus., 111 illus. in color. :) : online resource.
Edition Identifiers
- ISBN:9781493994243, 1493994247, 9781493994236, 1493994239, 9781493994250, 1493994255, 9781493994267, 1493994263
- DOI:10.1007/978-1-4939-9424-3
- oclc:on1228610945
- LC Classification:QD431-431.7
- Dewey Decimal:572.6
Table of Contents
Intersecting Stories of the Phagocyte NADPH Oxidase and Chronic Granulomatous Disease -- Mammalian NADPH Oxidases -- Enhanced Immunoaffinity Purification of Human Neutrophil Flavocytochrome B for Structure Determination by Electron Microscopy -- Purification and Characterization of DUOX Peroxidase Homology Domains (PHDs) -- A Close-Up View of the Impact of Arachidonic Acid on the Phagocyte NADPH Oxidase -- NOX5 Cell-Free Assay for the High-Throughput Screening of Small Molecules -- Spectroscopy of NOX Protein Family Members -- Soluble Regulatory Proteins for Activation of NOX Family NADPH Oxidases -- Insights into the NOX NADPH Oxidases Using Heterologous Whole Cell Assays -- The X-CGD PLB-985 Cell Model for NOX2 Structure-Function Analysis -- Functional Characterization of DUOX Enzymes in Reconstituted Cell Models -- Guidelines for the Detection of NADPH Oxidases by Immunoblot and RT-qPCR -- Methods for Detection of NOX-Derived Superoxide Radical Anion and Hydrogen Peroxide in Cells -- HPLC-Based Monitoring of Oxidation of Hydroethidine for Detection of NADPH Oxidase-Derived Superoxide Radical Anion -- Visualization of Intracellular Hydrogen Peroxide with the Genetically-Encoded …
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