Neurotrophic Factors: Methods and Protocols

by Skaper, Stephen D.

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Title Statements

Full title :Neurotrophic Factors : Methods and Protocols / edited by Stephen D. Skaper.

Summary

This volume collects protocols and procedures utilizing cellular, tissue, and whole animal models that can be applied to the investigation of neurotrophic factors and other agents impacting on these systems. It begins with chapters on the culture of neurons and glia from the central and peripheral nervous systems, neuron-glia and glia-glia co-culture models, oligodendrocytes, and cell-based assays for the evaluation of cell vitality. This revised second edition expands to cover methodology encompassing site-specific direct labeling of neurotrophins and their receptors, angiogenesis assays, stem cells, pancreatic beta-cells, axonal transport, synapse biology, dendritic spine analysis, and brain endothelial cells. Subsequent chapters are dedicated to in vivo lesion models of relevance to nervous system pathology, which can be applied to the investigation of neurotrophic factors and peptides, as well as protocols describing nanofiber- and nanoparticle-based methods for brain delivery of neurotrophic agents. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Neurotrophic Factors: Methods and Protocols, Second Edition is a valuable resource for a wide audience of readers as they explore nervous system function and pathology.

Edition Statements

Publication Date:2018.
Edition:2nd ed. 2018

Publication Statements

Publisher(s):Humana Press, Imprint, Springer New York
Place of Publication:New York, NY
Language:eng
Publication Date:2018.

The Physical Object

Description:1 online resource (XV, 458 pages 121 illustrations, 70 illustrations in color.) : : online resource.

Edition Identifiers

ISBN:9781493975716, 1493975714, 9781493975709, 1493975706
DOI:10.1007/978-1-4939-7571-6
oclc:on1058452157
LC Classification:RC321-580
Dewey Decimal:612.8

1. Neurotrophic Factors: An Overview.- Ocular Nerve Growth Factor (NGF) and NGF Eye Drop Application as Paradigms to Investigate NGF Neuroprotective and Reparative Actions -- Culture of Rodent Cortical, Hippocampal, and Striatal Neurons -- Culture of Neonatal Rodent Microglia, Astrocytes, and Oligodendrocytes from Cortex, Spinal Cord, and Cerebellum -- Central Nervous System Neuron-Glia Co-Culture Models and Application to Neuroprotective Agents -- Preparation of Adult Rat Sensory Neuron Cultures and Their Application to Growth Cone Turning Assays -- Sensory Neurons from Tau Transgenic Mice and Their Utility in Drug Screening -- Culture of Rat Mesencephalic Dopaminergic Neurons and Application to Neurotoxic and Neuroprotective Agents -- Culture of Neurons and Smooth Muscle Cells from the Myenteric Plexus of Adult Mice -- Astrocyte/Microglia Co-Cultures as a Model to Study Neuroinflammation -- Micropatterned Geometries Shape Oligodendrocyte and Microglia Plasticity -- Oligodendrocyte Progenitor Cell Cultures: A Model to Screen Neurotrophic Compounds for Myelin Repair -- Cell Enumeration Assays: Application of the MTT and Sulforhodamine B Assays to Lipopolysaccharide-Stimulated Neon…

Subjects

Biomedicine Laboratory Manual Laboratory manuals Manuels de laboratoire Médecine Medicine medicines (material) Nerve Growth Factors -- physiology Neurons -- physiology Neurosciences Receptors, Nerve Growth Factor