Neurotrophic factors: methods and protocols

by Skaper, Stephen D., (Stephen Drake)

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Title Statements

Full title :Neurotrophic factors : methods and protocols / edited by Stephen D. Skaper.

Summary

This volume collects protocols and procedures utilizing cellular, tissue, and whole animal models that can be applied to the investigation of neurotrophic factors and other agents impacting on these systems. It begins with chapters on the culture of neurons and glia from the central and peripheral nervous systems, neuron-glia and glia-glia co-culture models, oligodendrocytes, and cell-based assays for the evaluation of cell vitality. This revised second edition expands to cover methodology encompassing site-specific direct labeling of neurotrophins and their receptors, angiogenesis assays, stem cells, pancreatic beta-cells, axonal transport, synapse biology, dendritic spine analysis, and brain endothelial cells. Subsequent chapters are dedicated to in vivo lesion models of relevance to nervous system pathology, which can be applied to the investigation of neurotrophic factors and peptides, as well as protocols describing nanofiber- and nanoparticle-based methods for brain delivery of neurotrophic agents. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.uthoritative and practical, Neurotrophic Factors: Methods and Protocols, Second Edition is a valuable resource for a wide audience of readers as they explore nervous system function and pathology.

Edition Statements

Publication Date:©2018
Edition:Second edition

Publication Statements

Publisher(s):Humana Press
Place of Publication:New York, NY
Language:eng
Publication Date:©2018

The Physical Object

Description:1 online resource (xv, 458 pages) : : illustrations (some color)

Edition Identifiers

ISBN:9781493975716, 1493975714
DOI:10.1007/978-1-4939-7571-6
oclc:on1015341046
LC Classification:QP363
Dewey Decimal:573.8/536

Neurotrophic factors : an overview / Stephen D. Skaper -- Ocular nerve growth factor (NGF) and NGF eye drop application as paradigms to investigate NGF neuroportective and reparative actions / Paola Tirassa, Pamela Rosso, and Angela Iannitelli -- Culture of neonatal rodent cortical, hoppocampal, and striatal neurons / Laura Facci and Stephen D. Skaper -- Culture of neonatal rodent microglia, astrocytes, and oligodendrocytes from the cortex, spinal cord, and cerebellum ; Central nervous system neuron-glia co-culture models and application to neuroprotective agents / Stephen D. Skaper and Laura Facci -- Preparation of adult rat sensory neuron cultures and their application to growth cone turning assays / Christine A. Webber and Douglas W. Zochodne -- Sensory neurons from tau transgenic mice and their utility in drug screening / Aviva M. Tolkovsky and Jack Brelstaff -- Culture of rat mesencephalic dopaminergic neurons and application to neurotoxic and neuroprotective agents / Stephen D. Skaper, Massimo Barbierato, Vanni Ferrari, Morena Zusso, and Laura Facci -- Culture of neurons and smooth muscle cells from the myenteric plexus of adult mice / Paola Brun and Hamid I. Akbarali -- Ast…

Subjects

Amino Acids, Peptides, and Proteins Biological Factors Cell receptors Chemicals and Drugs Fonctions neurotrophiques -- Manuels de… Intercellular Signaling Peptides and Pr… Laboratory Manual Laboratory manuals Manuels de laboratoire Membrane Proteins Nerve Growth Factors Nerve Tissue Proteins Neurones -- Manuels de laboratoire. -- … Neurons -- Laboratory manuals. -- Physi… Neurons -- physiology Neurotrophic functions Neurotrophic functions -- Laboratory ma… Neurotrophines Peptides Produits biologiques