Nucleic acid aptamers: selection, characterization, and application

by Mayer, Günter Menger, Marcus M.

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Title Statements

Full title :Nucleic acid aptamers : selection, characterization, and application / edited by Günter Mayer, Marcus M. Menger.

Summary

This updated book reflects improvements in a variety of techniques used to study the aptamer field. Beginning with a section on selection procedures, the volume continues with methods to characterize aptamers’ interaction and structural properties by biophysical approaches, as well as a variety of applications that have been adapted to the aptamer compound class. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Nucleic Acid Aptamers: Selection, Characterization, and Application, Second Edition serves as an ideal guide for researchers aiming to further our understanding of aptamer biology and more.

Edition Statements

Publication Date:2023
Edition:Second edition

Publication Statements

Publisher(s):Humana Press
Place of Publication:New York, NY
Language:eng
Publication Date:2023

Notes

Includes index.

The Physical Object

Description:1 online resource (xi, 282 pages) : : illustrations (some color).

Edition Identifiers

ISBN:9781071626955, 1071626957
DOI:10.1007/978-1-0716-2695-5
oclc:on1346658844
LC Classification:QP625.O47
Dewey Decimal:572.84

Automated ssDNA SELEX Using Histidine Tagged Target Proteins -- Discovery of Aptamers against Cell Surface Markers Using Ligand-Guided Selection -- Implementation of Emulsion PCR for Amplification of Click-Modified DNA during SELEX -- Preparation of Chemically Modified DNA Library for SELEX via Incorporation of CLB-dUTP in Primer Extension Reaction -- RNA Capture-SELEX on Streptavidin Magnetic Beads -- The Bioinformatics of Aptamers: HT-SELEX Analysis with AptaSUITE -- Improvement of Aptamers by High-Throughput Sequencing of Doped-SELEX -- Analysis of Aptamer-Small Molecule Binding Interactions Using Isothermal Titration Calorimetry -- Determination of Aptamer Structure Using Circular Dichroism Spectroscopy -- Immobilization-Free Determination of Dissociation Constants Independent of Ligand Size Using MicroScale Thermophoresis -- Label-Free Determination of the Kinetic Parameters of Protein-Aptamer Interaction by Surface Plasmon Resonance -- Probing of Fluorogenic RNA Aptamers via Supramolecular Förster Resonance Energy Transfer with Universal Fluorescent Nucleobase Analog -- Slow-Off-Rate-Modified Aptamer Labeling for Fluorescence Microscopy and DNA-PAINT -- Aptamer-Field-Effect …