Oogenesis

Oogenesis: methods and protocols

by Nezis, Ioannis P.

Type Book
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This volume presents methods and techniques to study oogenesis in a broad range of organisms, from plants to mammals. Oogenesis: Methods and Protocols guides readers through protocols on models of developmental biology, oogenesis in plants, worms, fruit flies, mosquitos, butterflies, starfish, zebrafish, frog, chicken and mouse. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials…

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Title Statements

  • Full title :Oogenesis : methods and protocols / edited by Ioannis P. Nezis.

Summary

This volume presents methods and techniques to study oogenesis in a broad range of organisms, from plants to mammals. Oogenesis: Methods and Protocols guides readers through protocols on models of developmental biology, oogenesis in plants, worms, fruit flies, mosquitos, butterflies, starfish, zebrafish, frog, chicken and mouse. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Oogenesis: Methods and Protocols aims to ensure successful results in the further study of this vital field.

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Edition Statements

  • Publication Date:2016.

Publication Statements

  • Publisher(s):Humana Press
  • Place of Publication:New York, NY
  • Language:eng
  • Publication Date:2016.

Notes

Includes index.

The Physical Object

  • Description:1 online resource (x, 277 pages) : : illustrations

Edition Identifiers

  • ISBN:9781493937950, 1493937952
  • DOI:10.1007/978-1-4939-3795-0
  • oclc:ocn957580814
  • LC Classification:QL965
  • Dewey Decimal:571.8/45

Table of Contents

Histological Analysis of the Arabidopsis Gynoecium and Ovules Using Chloral Hydrate Clearing and Differential Interference Contrast Light Microscopy -- Indirect Immunofluorescence of Proteins in Oogenic Germ Cells of Caenorhabditis Elegans -- Antibody Staining In Drosophila Germaria -- In Vitro<Culturing and Live Imaging of Drosophila Egg Chambers: A History and Adaptable Method -- Using Fluorescent Reporters to Monitor Autophagy in the Female Germ Line Cells in Drosophila Melanogaster -- Analysis of Phagocytosis in the Drosophila Ovary -- Immuno-Gold Labeling Of Drosophila Follicles For Transmission Electron Microscopy -- Preparation of Drosophila Follicles for Transmission Electron Microscopy -- Quantitative Real-Time PCR Analysis of Gene Transcripts of Mosquito Follicles -- Studying Oogenesis in a Non-Model Organism Using Transcriptomics; Assembling, Annotating, and Analysing your Data -- Live Imaging of Centriole Dynamics by Fluorescently-Tagged Proteins un Starfish Oocyte Meiosis -- High-Pressure Freezing Electron Microscopy of Zebrafish Oocytes -- Cytoskeleton and Cytoskeleton-Bound RNA Visualization in Frog and Insect Oocytes -- RNA FISH, DNA FISH and Chromosome Painting of…

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