P-type ATPases

P-type ATPases: methods and protocols

by Bublitz, Maike

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This volume provides a broad collection of protocols for many of the common experimental techniques used for the characterization of P-type ATPases. P-Type ATPases: Methods and Protocols provides comprehensive practical instructions for all researchers in the P-type ATPase field, from the protein biochemist to the mouse geneticist, covering the identification and classification, isolation, purification, in vitro characterization, knock-out studies, as well as crystallization and structural anal…

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Title Statements

  • Full title :P-type ATPases : methods and protocols / edited by Maike Bublitz, Department of Biochemistry, University of Oxford, Oxford, UK

Summary

This volume provides a broad collection of protocols for many of the common experimental techniques used for the characterization of P-type ATPases. P-Type ATPases: Methods and Protocols provides comprehensive practical instructions for all researchers in the P-type ATPase field, from the protein biochemist to the mouse geneticist, covering the identification and classification, isolation, purification, in vitro characterization, knock-out studies, as well as crystallization and structural analysis. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, P-Type ATPases: Methods and Protocols aims to ensure successful results in the further study of this vital field of research

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Edition Statements

  • Publication Date:2016

Publication Statements

  • Publisher(s):Humana Press
  • Place of Publication:New York, NY
  • Language:eng
  • Publication Date:2016

Notes

Includes index

The Physical Object

  • Description:xiv, 544 Seiten : Illustrationen

Edition Identifiers

  • ISBN:9781493931798, 1493931792, 9781493931781, 1493931784
  • oclc:ocn936134295
  • LC Classification:QP609.A3
  • Dewey Decimal:572/.7553

Table of Contents

An Introduction to P-type ATPase researchPurification of Na, K-ATPase from Pig Kidney -- Preparation of Ca2+-ATPase1a Enzyme from Rabbit Sarcoplasmic Reticulum -- Isolation of H+/K+-ATPase-enriched Membrane Fraction from Pig Stomachs -- Overproduction of PiB-type ATPases -- Coordinated Overexpression in Yeast of a P4-ATPase and its Associated Cdc50 Subunit: The Case of the Drs2p/Cdc50p Lipid Flippase Complex -- The Plasma Membrane Ca2+-ATPase: Purification by Calmodulin Affinity Chromatography, and Reconstitution of the Purified Protein -- Expression of Na, K-ATPase and H, K-ATPase Isoforms With The Baculovirus Expression System -- Time-dependent Protein Thermostability Assay -- Colorimetric Assays of Na, K-ATPase -- ATPase Activity Measurements by an Enzyme-Coupled Spectrophotometric Assay -- Antimony-Phosphomolybdate ATPase Assay -- ATPase Activity Measurements using radiolabeled ATP -- Assaying P-type ATPases Reconstituted in Liposomes -- Coupling ratio for Ca2+ Transport by Calcium Oxalate Precipitation -- Calcium Uptake in Crude Tissue Preparation -- Measuring H+ Pumping and Membrane Potential Formation in Sealed Membrane Vesicle Systems -- Assay of Flippase Activity in Prote…

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Subjects

Adenosine triphosphatase -- Laboratory … Adénosine-triphosphatase Adénosine-triphosphatase -- Manuels de … Laboratory manuals

Writers

Bublitz, Maike

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