Pathogenic Yersinia: methods and protocols

by Lawrenz, Matthew B. Vadyvaloo, Viveka

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Title Statements

Full title :Pathogenic Yersinia : methods and protocols / edited by Viveka Vadyvaloo, Matthew B. Lawrenz.

Summary

"This volume delivers a compendium of detailed protocols to the research community in order to aid in the investigation and discovery of Yersinia virulence mechanisms using important in vivo and in vitro infection models, which have led to major advances in the field and in our understanding of Yersinia pathogen-host interactions. Beginning with a section on mouse models, the book continues with chapters covering the monitoring of bacteria during infection, invertebrate models, as well as Yersinia interaction with immune cells and immune signaling. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. " -- Prové de l'editor.

Edition Statements

Publication Date:2019

Publication Statements

Publisher(s):Humana Press
Place of Publication:New York, NY
Language:eng
Publication Date:2019

Notes

Conté: 1. Media culturing methods for selection and biochemical identification of specific Yersinia phenotypes 2. Plague Vertebrate Infection models: Intradermal inoculation 3. Plague Vertebrate Infection models Intranasal inoculation 4. Plague Vertebrate Infection models Aerosol inoculation 5. Plague Vertebrate Infection models Septicemic inoculation 6. Plague Vertebrate Infection models Flea-borne transmission 7. Plague Invertebrate Infection model: Caenorhabditis elegans 8. Plague Invertebrate Infection models: Flea Model 9. Plague Invertebrate Infection models: Drosophila Model 10. Yersiniosis Infection models: Oral gavage 11. Immune cell infection model: Macrophage model 12. Immune cell infection model: Neutrophil model 13. Intravital microscopy of early bubonic plague events 14. Bioluminescence imaging to track bacterial colonization and dissemination 15. Mutagenesis of the Yersinia spp 16. Dual tissue RNAseq 17. Thermosensor RNA regulation 18. The Type III secretion system (T3SS): in vitro 19. The Type III secretion system (T3SS): in vitro 20. In vivo gene expression 21. Metal studies for plague 22. The Yersinia Inflammasome 23. Proteomic Bioforensic detection methods.