PCR technology

PCR technology: current innovations

by Bustin, Stephen A. Nolan, Tania

Type Book
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A technique used to amplify the number of copies of a specific region of DNA, the polymerase chain reaction (PCR) is at the forefront of the dramatic development of biochemistry. This text provides the tools for developing innovative approaches to using this leading technology. It includes theoretical considerations, discussions, and a selection of state-of-the-art techniques for mutation studies, clinical diagnosis, and the detection of food-borne pathogens. This edition also discusses the pre…

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Title Statements

  • Full title :PCR technology : current innovations / edited by Tania Nolan, Stephen A. Bustin.

Summary

A technique used to amplify the number of copies of a specific region of DNA, the polymerase chain reaction (PCR) is at the forefront of the dramatic development of biochemistry. This text provides the tools for developing innovative approaches to using this leading technology. It includes theoretical considerations, discussions, and a selection of state-of-the-art techniques for mutation studies, clinical diagnosis, and the detection of food-borne pathogens. This edition also discusses the preparation of PCR experiments, includes examples of analytical PCR divided into qualitative and quantitative applications, and explores preparative methods that address DNA generation for further analysis and in vitro evolution--Provided by publisher.

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Edition Statements

  • Publication Date:2013.
  • Edition:3rd ed

Publication Statements

  • Publisher(s):Taylor & Francis
  • Place of Publication:Boca Raton
  • Language:eng
  • Publication Date:2013.

Notes

Description based upon print version of record.

The Physical Object

  • Description:1 online resource (457 p.)

Edition Identifiers

  • ISBN:0429110456, 9780429110450, 1439848130, 9781439848135, 9781439848050, 143984805X
  • DOI:10.1201/b14930
  • oclc:on1290070641
  • LC Classification:QP606.D46
  • Dewey Decimal:572.8/636

Table of Contents

Pt. 1. Template preparation -- pt. 2. Reaction components -- pt. 3. Instruments -- pt. 4. Design, optimization, QC, and standardization -- pt. 5. Data analysis -- pt. 6. Applications.

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Subjects

ADN polymérases DNA polymerases Nucleic acids -- Analysis Polymerase Chain Reaction -- methods Transférases

Writers

Bustin, Stephen A. Nolan, Tania

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