Periodontal pathogens: methods and protocols

by Hasegawa, Yoshiaki Nagano, Keiji

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Title Statements

Full title :Periodontal pathogens : methods and protocols / edited by Keiji Nagano, Yoshiaki Hasegawa.

Summary

This book addresses the major periodontal pathogens implicated as causal agents in periodontal disease, including Porphyromonas gingivalis, Tannerella forsythia, Treponema denticola, Fusobacterium nucleatum, Aggregatibacter actinomycetemcomitans, and Prevotella spp. Beginning with methods for bacterial genetic manipulation, the volume continues with sections on experimental methods to examine virulence factors, interactions with other pathogenic microorganism and host cells, as well as a chapter on an animal model of periodontitis. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Periodontal Pathogens: Methods and Protocols serves as an extensive and useful reference for researchers studying periodontal pathogens and will help elucidate the causes of periodontal disease and the systemic diseases related to it.

Edition Statements

Publication Date:2021

Publication Statements

Publisher(s):Humana Press
Place of Publication:New York, NY
Language:eng
Publication Date:2021

Notes

Part I: Methods for Bacterial Genetic Manipulation 1. Site-Directed and Random Mutagenesis in Porphyromonas gingivalis: Construction of Fimbriae-Related-Gene Mutant So-ichiro Nishiyama, Yoshiaki Hasegawa, and Keiji Nagano 2. Genetic Manipulations of Oral Spirochete Treponema denticola Kurni Kurniyati and Chunhao Li 3. Construction of a Gene-Deletion Mutant in Tannerella forsythia Keiji Nagano and Yoshiaki Hasegawa 4. Construction of a Mutant in Prevotella melaninogenica Using the Conjugation Transfer Method with Escherichia coli Yoshio Kondo 5. Genetic Transformation of Fusobacterium nucleatum Akihiro Yoshida and Akihiko Ikegami Part II: Experimental Methods to Examine Virulence Factors 6. Genotyping of Porphyromonas gingivalis in Relationship to Virulence Atsuo Amano, Youn-Hee Choi, and Hiroki Takeuchi 7. Transport and Polymerization of Porphyromonas gingivalis Type V Pili Mikio Shoji, Satoshi Shibata, Mariko Naito, and Koji Nakayama 8.

Purification of Native Mfa1 Fimbriae from Porphyromonas gingivalis Yoshiaki Hasegawa, Keiji Nagano, Yukitaka Murakami, and Richard J. Lamont 9. Crystallization of Recombinant Fimbrial Proteins of Porphyromonas gingivalis Thomas Heidler and Karina Persson 10. Enzymatic Characteristics and Activities of Gingipains from Porphyromonas gingivalis Tomoko Kadowaki 11. Structural Characterization of the Type IX Secretion System in Porphyromonas gingivalis Dhana G. Gorasia, Eric Hanssen, Paul D. Veith, and Eric C. Reynolds 12. Methods for Functional Characterization of the Type IX Secretion System of Porphyromonas gingivalis Keiko Sato 13. Purification of Tannerella forsythia Surface (S-) Layer Proteins Sreedevi Chinthamani, Prasad R. Settem, Kiyonobu Honma, Takuma Nakajima, and Ashu Sharma 14. Separation of Glycosylated OmpA-Like Proteins from Porphyromonas gingivalis and Tannerella forsythia Yukitaka Murakami, Keiji Nagano, and Yoshiaki Hasegawa 15.

Intranasal Vaccine Study Using Porphyromonas gingivalis Membrane Vesicles: Isolation Method and Application to a Mouse Model Satoru Hirayama and Ryoma Nakao 16. Analysis of the Butyrate-Producing Pathway in Porphyromonas gingivalis Yasuo Yoshida 17. Characterization of the Treponema denticola Virulence Factor Dentilisin Yuichiro Kikuchi and Kazuyuki Ishihara 18. Evaluation of the Virulence of Aggregatibacter actinomycetemcomitans through the Analysis of Leukotoxin Toshiyuki Nagasawa, Satsuki Kato, and Yasushi Furuichi 19. Lipoprotein Extraction from Microbial Membrane and Lipoprotein/Lipopeptide Transfection into Mammalian Cells Akira Hasebe, Ayumi Saeki, and Ken-ichiro Shibata Part III: Interactions with Other Pathogenic Microorganism and Host Cells 20. Analysis of the Interaction between HIV and Periodontopathic Bacteria that Reactivates HIV Replication in Latently Infected Cells Kenichi Imai 21.

Invasion of Gingival Epithelial Cells by Porphyromonas gingivalis Hiroki Takeuchi and Atsuo Amano 22. Analysis of Interaction between Porphyromonas gingivalis and Endothelial Cells In Vitro Kenji Matsushita Part IV: Animal Model of Periodontitis 23. Analysis of Experimental Ligature-Induced Periodontitis Model in Mice Hikaru Tamura, Tomoki Maekawa, Takumi Hiyoshi, and Yutaka Terao.

The Physical Object

Description:xi, 256 pages : : illustrations ; : 27 cm

Edition Identifiers

ISBN:1071609386, 9781071609385
oclc:on1154813215
LC Classification:RK361
Dewey Decimal:617.6/32

Subjects

Bacteroidetes -- pathogenicity Disease Models, Animal Diseases -- Animal models Laboratory Manual Laboratory manuals Maladies -- Modèles animaux Manuels de laboratoire Parodontopathies Periodontal disease Periodontal disease -- Laboratory manua… Periodontal Diseases