Phase-separated biomolecular condensates: methods and protocols

by Banerjee, Priya R. Spille, Jan-Hendrik Zhou, Huan-Xiang

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Title Statements

Full title :Phase-separated biomolecular condensates : methods and protocols / edited by Huan-Xiang Zhou, Jan-Hendrik Spille, Priya R. Banerjee.

Summary

This volume provides readers with a broad collection of theoretical, computational, and experimental methods to quantitatively study the properties of phase-separate biomolecular condensates in diverse systems. The chapters in this book cover topics such as theoretical and computational methods; methods for in vitro characterization of biomolecular condensates; and techniques that enable in-cell characterization of biomolecular condensates. Written in the highly successful Methods in Molecular Biology series format, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and expert tips on troubleshooting and avoiding known pitfalls. Comprehensive and thorough, Phase-Separated Biomolecular Condensates: Methods and Protocols is a valuable resource that helps researchers learn and use established methods to study both biophysical properties and biological functions of biomolecular condensates. .

Edition Statements

Publication Date:2023

Publication Statements

Publisher(s):Humana Press
Place of Publication:New York, NY
Language:eng
Publication Date:2023

Notes

Includes index.

The Physical Object

Description:1 online resource (xiv, 449 pages) : : illustrations.

Edition Identifiers

ISBN:9781071626634, 1071626639
DOI:10.1007/978-1-0716-2663-4
oclc:on1348179325
LC Classification:QH581.2
Dewey Decimal:571.6/5

Calculating Binodals and Interfacial Tension of Phase-Separated Condensates from Molecular Simulations, with Finite-Size Corrections -- Field-Theoretic Simulation Method to Study the Liquid-Liquid Phase Separation of Polymers -- Numerical Techniques for Applications of Analytical Theories to Sequence-Dependent Phase Separations of Intrinsically Disordered Proteins -- An Introduction to the Stickers-and-Spacers Framework as Applied to Biomolecular Condensates -- Multi-Scale Modeling of Protein-RNA Condensation In and Out of Equilibrium -- Fluorescence Lifetime Imaging Microscopy of Biomolecular Condensates -- Single-Molecule Fluorescence Methods to Study Protein-RNA Interactions Underlying Biomolecular Condensates -- Fluorescence Correlation Spectroscopy and Phase Separation -- Measurement of Protein and Nucleic Acid Diffusion Coefficients within Biomolecular Condensates Using In-Droplet Fluorescence Correlation Spectroscopy -- Single-Molecule Imaging of the Phase Separation-Modulated DNA Compaction to Study Transcriptional Repression -- Phase Separation-Based Biochemical Assays for Biomolecular Interactions -- Determining Thermodynamic and Material Properties of Biomolecular Conde…