Phloem: Methods and Protocols

by Liesche, Johannes

Type Book

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Title Statements

Full title :Phloem : Methods and Protocols / edited by Johannes Liesche.

Summary

This detailed book presents methods that enable the determination of parameters relevant to phloem research in the most efficient ways, putting plant scientists with access to adequate instrumentation in the position to answer any phloem-related question. The collection explores techniques that have been used for decades, such as tracing phloem transport with carbon isotopes, as well as recent developments, such as esculin-based assays of phloem transport and super-resolution microscopy of phloem proteins. As such, the book presents the state-of-the-art in phloem research and, at the same time, a starting point for the development of new methods that will fill the remaining gaps in the phloem researcher's toolbox in the future. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and comprehensive, Phloem: Methods and Protocols is an ideal guide for all researchers exploring this challenging area of plant biology.

Edition Statements

Publication Date:2019.
Edition:1st ed. 2019

Publication Statements

Publisher(s):Humana, Imprint, Springer New York
Place of Publication:New York, NY
Language:eng
Publication Date:2019.

The Physical Object

Description:1 online resource (XIV, 473 pages 107 illustrations, 87 illustrations in color.) : : online resource

Edition Identifiers

ISBN:9781493995622, 1493995626, 9781493995615, 1493995618, 9781493995639, 1493995634, 9781493995646, 1493995642
DOI:10.1007/978-1-4939-9562-2
oclc:on1115075933
LC Classification:QH301-705
Dewey Decimal:580

Part 1 : Investigating phloem anatomy -- Optimal preparation of tissue sections for light-microscopic analysis of phloem anatomy -- Transmission electron microscopy of the phloem with minimal artifacts -- Scanning electron microscopy of the phloem -- Non-invasive investigation of phloem structure by 3D synchrotron X-ray microtomography -- Quantification of leaf phloem anatomical features with microscopy -- Methods of phloem visualization -- a clear future in sight? -- Part 2 : Visualizing phloem proteins -- Super-resolution microscopy of phloem proteins -- Live-cell imaging of fluorescently-tagged phloem proteins with confocal microscopy -- Immunohistochemical localization of proteins in the phloem: problems and solutions -- Part 3 : Sampling phloem sap -- SStudying phloem loading with EDTA-facilitated phloem exudate collection and analysis -- Measurement of inner bark and leaf osmolality -- Using 13C to quantify phloem transport on tall plants in the field -- Non-invasive determination of phloem transport speed with carbon-14 (14C) -- Detecting rapid changes in carbon transport and partitioning with carbon-11 (11C) -- In vivo veritas: radiotracers in studies of phloem transport o…