piRNA: methods and protocols

by Iwasaki, Yuka W. Parrish, Nicholas F.

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Title Statements

Full title :piRNA : methods and protocols / edited by Nicholas F. Parrish, Yuka W. Iwasaki.

Summary

This detailed volume explores newly-developed methods in PIWI-interacting RNAs (piRNAs) research, methods currently applied to other ncRNAs involved in nuclear regulation which can be used to study piRNAs, and piRNA methods applied in non-classical organisms. It also includes several bioinformatic and biophysical methods related to piRNA studies, consistent with the increasing importance of high-throughput sequencing and computational methods. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, piRNA: Methods and Protocols serves as an ideal guide for researchers seeking to elucidate the numerous mysteries of this area of multicellular biology.

Edition Statements

Publication Date:2022

Publication Statements

Publisher(s):Humana Press
Place of Publication:New York, NY
Language:eng
Publication Date:2022

The Physical Object

Description:1 online resource (1 PDF file (xii, 397 pages)) : : illustrations (chiefly color).

Edition Identifiers

ISBN:9781071623800, 107162380X
DOI:10.1007/978-1-0716-2380-0
oclc:on1335769919
LC Classification:QP623.5.S63
Dewey Decimal:572.8/8

Functional Analysis of Individual piRNAs in Aedes aegypti Cells and Embryos Using Antisense Oligonucleotides -- CRISPR-Mediated Genome Engineering in Aedes aegypti -- PIWI-Directed DNA Elimination for Tetrahymena Genetics -- Planarian PIWI-piRNA Interaction Analysis Using Immunoprecipitation and piRNA-Sequencing -- Isolation and Processing of Bovine Oocytes for Small RNA Sequencing -- 3D Imaging and In Situ Hybridization for Uncovering the Functions of MicroRNA in Rice Anther -- Cloning, Sequencing, and Linkage Analysis of piRNAs -- Drosophila Genetic Resources for Elucidating piRNA Pathway -- Generation of Stable Drosophila Ovarian Somatic Cell Lines Using the piggyBac System -- Whole Mount RNA FISH Combined with Immunofluorescence for Analysis of the Telomeric Ribonucleoproteins in the Drosophila Germline -- CRISPR-Mediated Activation of Transposable Elements in Embryonic Stem Cells -- Method for Evaluating Effects of Non-Coding RNAs on Nucleosome Stability -- Revisiting the Glass Treatment for Single Molecule Analysis of ncRNA Function -- Low Input Genome-Wide DNA Methylation Analysis with Minimal Library Amplification -- Solid-Support Directional (SSD) RNA-Seq as a Companion M…