Plant innate immunity: methods and protocols

by Gassmann, Walter

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Title Statements

Full title :Plant innate immunity : methods and protocols / edited by Walter Gassmann.

Summary

Back Cover Copy The volume presents valuable methods that look at important biological processes not traditionally assayed in the study of plant immunity, and at non-model systems. The chapters in this book cover topics such as identifying host targets of acetylating effectors by immunoprecipitation; quantifying ATP release from plant cells; protein-DNA interactions; DNA methylation; measurement and playback of leaf vibrations; natural infection routes of Xanthomonas campestris pv. campestris using Arabidopsis; and isolating favorable plant-growth-promoting bacteria from the phytosphere. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Plant Innate Immunity: Methods and Protocols is an essential resource for all researchers interested in expanding their knowledge and learning new techniques in this ever-growing field.

Edition Statements

Publication Date:©2019

Publication Statements

Publisher(s):Humana Press
Place of Publication:New York, NY
Language:eng
Publication Date:©2019

The Physical Object

Description:1 online resource (xiii, 260 pages) : : illustrations (some color)

Edition Identifiers

ISBN:9781493994588, 1493994581
DOI:10.1007/978-1-4939-9458-8
oclc:on1100792573
LC Classification:SB750
Dewey Decimal:580

Using Bioinformatics and Molecular Biology to Streamline Construction of Effector Libraries for Phytopathogenic Pseudomonas syringae Strains -- Immunoprecipitation Under Non-Denaturing or Denaturing Conditions of Lysine-Acetylated Proteins Expressed in planta -- Protein Acetylation in Pathogen Virulence and Host Defense: In Vitro Detection of Protein Acetylation by Radiolabeled Acetyl CoenzymeA -- A Novel Assay Based on Confocal Microscopy to Test for Pathogen Silencing Suppressor Functions -- Quantification of Extracellular ATP in Plant Suspension Cell Cultures -- Measuring Pectin Properties to Track Cell Wall Alterations during Plant-Pathogen Interactions -- Method to Study Dynamics of Membrane-Bound Plant Transcription Factors during Biotic Interactions in Tomato -- Preparation of Plant Material for Analysis of Protein-Nucleic Acid Interactions by FRET-FLIM -- Analysis of DNA Methylation Profile in Plants by Chop-PCR -- Change in Nucleosome Dynamics during Stress Responses in Plants -- Measuring Cell Ploidy Level in Arabidopsis thaliana by Flow Cytometry -- A Method for Investigating the Pseudomonas syringae-Arabidopsis thaliana Pathosystem under Various Light Environments -- I…

Subjects

Botany Botany & plant sciences Plant immunology Plantes -- Immunologie Plantes -- Résistance aux maladies et f… Plants -- Disease and pest resistance SCIENCE -- Life Sciences