Plant lipids: methods and protocols

by Bartels, Dorothea Dörmann, Peter

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Title Statements

Full title :Plant lipids : methods and protocols / edited by Dorothea Bartels, Peter Dörmann.

Summary

This volume explores analytical methods to study complex lipid mixtures from plants and algae. The chapters in this book are organized into five parts and cover topics such as basic methods of lipid isolation and analysis; mass spectrometry and NMR analysis; lipid isolation and analysis from plant tissues, cell compartments and organelles; lipid signaling, lipid-protein interactions, and imaging; and lipid databases. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Plant Lipids: Methods and Protocols is a valuable guide for experienced researchers and undergraduate, graduate, and Ph. D. students. This book is also an excellent resource for novice scientists with little to no experience in lipid experiments who are interested in approaching this field experimentally.

Edition Statements

Publication Date:2021

Publication Statements

Publisher(s):Humana Press
Place of Publication:New York, NY
Language:eng
Publication Date:2021

Notes

Includes index.

The Physical Object

Description:1 online resource (xiv, 472 pages) : : illustrations (some color)

Edition Identifiers

ISBN:9781071613627, 1071613626
DOI:10.1007/978-1-0716-1362-7
oclc:on1253758511
LC Classification:QK898.L56
Dewey Decimal:572/.572

Lipid Isolation from Plants -- Three Methods to Extract Membrane Glycerolipids: Comparing Sensitivity to Lipase Degradation and Yield -- Thin-Layer Chromatography -- Lipid Analysis by Gas Chromatography and Gas Chromatography Mass Spectrometry -- 14C-Tracing of Lipid Metabolism -- Methods of Lipid Analyses for Microalgae: Charophytes, Eustigmatophytes, and Euglenophytes -- Direct Infusion Mass Spectrometry for Complex Lipid Analysis -- Fatty Acid Composition by Total-Acyl-Lipid--Collision Induced Dissociation--Time-of-Flight (TAL-CID-TOF) Mass Spectrometry -- Targeted Analysis of the Plant Lipidome by UPLC-NanoESI-MS/MS -- Mass Spectrometry-Based Profiling of Plant Sphingolipids from Typical and Aberrant Metabolism -- Analysis of Free and Esterified Sterol Content and Composition in Seeds using GC and ESI-MS/MS -- Techniques for the Measurement of Molecular Species of Acyl-CoA in Plants and Microalgae -- Quantification of Acyl-Acyl Carrier Proteins for Fatty Acid Synthesis using LC-MS/MS -- Structural Analysis of Glycosylglycerolipids using NMR Spectroscopy -- Analysis of Extracellular Cell Wall Lipids: Wax, Cutin and Suberin in Leaves, Roots, Fruits, and Seeds -- Isolation of Lip…