Pluripotent stem cells

Pluripotent stem cells: methods and protocols

by Lakshmipathy, Uma Vemuri, Mohan C.

Type Book
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Human pluripotent stem cells such as human embryonic stem cells (hESC) and induced pluripotent stem cells (iPSC) with their unique developmental plasticity hold immense potential as cellular models for drug discovery and in regenerative medicine as a source for cell replacement. While hESC are derived from a developing embryo, iPSC are generated with forced expression of key transcription factors to convert adult somatic cells to ESC-like cells, a process termed reprogramming. Using iPSC overco…

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Title Statements

  • Full title :Pluripotent stem cells : methods and protocols / edited by Uma Lakshmipathy, Mohan C. Vemuri.

Summary

Human pluripotent stem cells such as human embryonic stem cells (hESC) and induced pluripotent stem cells (iPSC) with their unique developmental plasticity hold immense potential as cellular models for drug discovery and in regenerative medicine as a source for cell replacement. While hESC are derived from a developing embryo, iPSC are generated with forced expression of key transcription factors to convert adult somatic cells to ESC-like cells, a process termed reprogramming. Using iPSC overcomes ethical issues concerning the use of developing embryos and it can be generated from patient-specific cells for downstream applications. Pluripotent Stem Cells: Methods and Protocols highlights the best methods and systems for the entire work flow. Divided into four convenient sections, topics include a focus on producing iPSC from diverse somatic sources, media systems for expanding ESC and iPSC with detailed protocols for directed differentiation into specific lineages, commonly used cellular and molecular characterization methods, and the potential application of labeled stem cells with specific methods for cloning, gene delivery and cell engineering. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Pluripotent Stem Cells: Methods and Protocols seeks to serve both professionals and novices with its well-honed methodologies in an effort to further our knowledge of this essential cellular feature.

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Edition Statements

  • Publication Date:©2013

Publication Statements

  • Publisher(s):Humana Press
  • Place of Publication:New York
  • Language:eng
  • Publication Date:©2013

The Physical Object

  • Description:1 online resource (x, 292 pages) : : illustrations (some color)

Edition Identifiers

  • ISBN:9781627033480, 1627033483
  • DOI:10.1007/978-1-62703-348-0
  • oclc:ocn837693702
  • LC Classification:QH588.S83
  • Dewey Decimal:616.02/774

Subjects

Celbiologie Cellular Biology Cellules souches embryonnaires Cellules souches multipotentes Embryonic stem cells Internet resources Laboratory manuals Multipotent Stem Cells Pluripotent Stem Cells stamcellen Stem cells

Writers

Lakshmipathy, Uma Vemuri, Mohan C.

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