Practical capillary electrophoresis

High performance capillary electrophoresis (HPCE) is the newest and perhaps most powerful separation technique available today. This single-authored text provides an integrated, comprehensive, and clearly illustrated look at the field. Users of HPCE will gain a basic understanding of principles underlying electrophoresis and go on to learn about mode selection, methods development, detection, and quantative analysis. Ideally suited for analytical chemists and analytical biochemists with applications involving small molecules, proteins, peptides, DNA, and ion separations, this book provides a comparative assessment of related techniques. The author is an internationally recognised scientist and serves as the instructor for short courses on HPCE as offered by the American Chemical Society. * Stresses basic principles and applications * Helps users select appropriate HPCE modes and develop methods * Describes how to perform quantitative analyses * reinforces concepts with clear illustrations.

Introduction -- Electrophoresis -- Microchromatographic separation methods -- Capillary electrophoresis -- Historical perspective -- Instrumentation -- Sources of information on HPCE -- Capillary electrophoresis: a family ot techniques -- Basic concepts -- Electrical conduction in fluid solution -- Language of electrophoresis -- Electroendoosmosis -- Efficiency -- Resolution -- Joule heating -- Optimizing the voltage and temperature -- Capillary diameter and buffer ionic strength -- Optimizing the capillary length -- Buffers -- Capillaries -- Capillary zone electrophoresis -- Mobility -- Buffer additives -- Solute-wall interactions -- Bandbroadening -- Applications -- Methods development -- Capillary isoelectric focusing -- Capillary coatings and electroosmotic flow -- Performing a run -- Focusing and mobilization problems -- Applications -- Size separations in capillary gels and polymer networks -- Separation mechanism -- Materials for CGE -- Applications -- Pulsed-field capillary electrophoresis -- Future of CGE -- Capillary isotachophoresis -- Separation mechanism -- Instrumental aspects -- Buffer selection.

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