Practical fluorescence spectroscopy

Presenting a detailed, hands-on approach to fluorescence spectroscopy, this book describes experiments that cover basic spectroscopy and advanced aspects of fluorescence spectroscopy. It emphasizes practical guidance, providing background on fundamental concepts as well as guidance on how to handle artifacts, avoid common errors, and interpret data. Nearly 150 experiments from biophysics, biochemistry, and the biomedical sciences demonstrate how methods are applied in practical applications. The result is a hands-on guide to the most important aspects of fluorescence spectroscopy, from steady-state fluorescence to advanced time-resolved fluorescence. Provides a complete overview of nearly 150 experiments using fluorescence spectroscopy, from basic to advanced applications Presents laboratory methods using a variety of instrumental setups with detailed discussion of data analysis and interpretations Covers steady-state phenomena, time-resolved phenomena, and advanced methods Spans biophysical, biochemical, and biomedical applications Describes related concepts, theory, and mathematical background as well as commercially available instruments used for measurements

Cover -- Half Title -- Title Page -- Copyright Page -- Table of Contents -- Preface -- Authors -- Chapter 1: Theory of Light and Light Interaction with Matter -- 1-1 BASICS OF LIGHT -- 1-2 LIGHT POLARIZATION -- Polarizers -- 1-3 INTERACTION OF LIGHT AND CHROMOPHORES -- Absorption of Light -- Emission of Light -- 1-4 INTERACTIONS AMONG CHROMOPHORES -- Fluorescence Quenching -- Fluorescence (Förster) Resonance Energy Transfer (FRET) -- 1-5 SCATTERING -- Elastic Scattering -- Raman Scattering -- SUMMARY -- Chapter 2: Experimental Basics -- 2-1 TYPES OF CUVETTES -- 2-2 TYPES OF FILTERS

2-3 MEASURING TRANSMITTANCE/ABSORBANCE -- Special Cases of Measuring Absorption -- Measurements of Absorption of Low Volume Samples -- Measurement of Absorption of Small (Solid) Samples -- 2-4 FACTORS AFFECTING THE PRECISION OF ABSORPTION MEASUREMENT -- 2-5 EFFECT OF SAMPLE SCATTERING ON MEASURED TRANSMISSION AND ABSORBANCE -- 2-6 MEASURING EMISSION -- 2-7 EFFICIENCY OF COLLECTING EMISSION: MEASURING QUANTUM YIELD -- Quantum Yield Calculation -- How to Convert from Wavenumbers to Wavelengths? -- Absorption -- Area under the Emission Spectra -- 2-8 WAVELENGTH CALIBRATION -- 2-9 INSTRUMENT RESPONSE

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