Protein folding: methods and protocols

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Title Statements

Full title :Protein folding : methods and protocols / edited by Victor Muñoz.

Summary

This volume provides comprehensive protocols on experimental and computational methods that are used to study probe protein folding reactions and mechanisms. Chapters divided into five parts detail protein engineering, protein chemistry, experimental approaches to investigate the thermodynamics and kinetics of protein folding transitions, probe protein folding at the single molecule, analysis and interpretation of computer simulations, procedures and tools for the prediction of protein folding properties. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Protein Folding: Methods and Protocols aims to be a useful practical guide to researches to help further their study in this field.

Edition Statements

Publication Date:2022

Publication Statements

Publisher(s):Humana Press
Place of Publication:New York, NY
Language:eng
Publication Date:2022

The Physical Object

Description:xi, 419 pages : : illustrations (some color) ; : 26 cm.

Edition Identifiers

ISBN:9781071617151, 107161715X
oclc:on1246353573
LC Classification:QP551
Dewey Decimal:572.633

Mutational Analysis of Protein Folding Transition States: Phi-values -- Engineered Metal-Binding Sites to Probe Protein Folding Transition States: Psi Analysis -- Site-Specific Interrogation of Protein Structure and Stability -- Purification and Handling of the Chaperonin GroEL -- Folding free energy surfaces from Differential Scanning Calorimetry -- Fast Folding Kinetics using Nanosecond Laser-Induced Temperature Jump Methods7. Measurement of Submillisecond Protein Folding using Trp Fluorescence and Photochemical Oxidation -- Native State Hydrogen Exchange-Mass Spectrometry Methods to Probe Protein Folding and Unfolding -- Multi-Probe Equilibrium Analysis of Gradual (Un)Folding Processes -- Nmr Analysis Of Protein Folding Interaction Networks -- NMR Relaxation Dispersion Methods for the Structural and Dynamic Analysis of Quickly Interconverting, Low-Populated Conformational Sub-States -- Native State Hydrogen Exchange-Mass Spectrometry Methods to Probe Protein Folding and Unfolding -- Single-Molecule Fluorescence Spectroscopy Approaches for Probing Fast Biomolecular Dynamics and Interactions -- Theory and Analysis of Single-Molecule FRET experiments -- Mechanochemical Evolution …