Protein-ligand interactions: methods and applications

by Daviter, Tina Johnson, Christopher M. McLaughlin, Stephen H. Williams, Mark A.,, Dr

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Title Statements

Full title :Protein-ligand interactions : methods and applications / edited by Tina Daviter, Christopher M. Johnson, Stephen H. McLaughlin, Mark A. Williams.

Summary

This third edition provides new and updated chapters detailing a complete introduction to common and emerging procedures for characterizing the interactions of individual proteins with their natural ligands, drugs or other binding partners. Chapters detail natural substrates, potential drug leads, quantitative understanding of the mechanism of interaction, and different techniques. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Protein Ligand Interactions: Methods and Applications, Third Edition serves as an ideal guide for researchers new to the field of biophysical characterization of protein interactions.

Edition Statements

Publication Date:2021
Edition:Third edition

Publication Statements

Publisher(s):Humana Press
Place of Publication:New York, NY
Language:eng
Publication Date:2021

Notes

Includes index.

The Physical Object

Description:1 online resource (xi, 492 pages) : : illustrations (some color)

Edition Identifiers

ISBN:9781071611975, 1071611976
DOI:10.1007/978-1-0716-1197-5
oclc:on1247938121
LC Classification:QP551
Dewey Decimal:572/.6

Assessing and Improving Protein Sample Quality -- A Familiar Protein-Ligand Interaction Revisited with Multiple Methods -- Interactions of a Signal Transduction Protein Investigated by Fluorescence Stopped-Flow Kinetics -- Kinetic Methods of Deducing Binding Mechanisms Involving Intrinsically Disordered Proteins -- Isothermal Titration Calorimetry -- Measuring the Kd of Protein-Ligand Interactions Using Microscale Thermophoresis -- Quartz Crystal Microbalance with Dissipation Monitoring (QCM-D); Preparing Functionalized Lipid Layers for the Study of Complex Protein-Ligand Interactions -- Indirect Detection of Ligand Binding by Thermal Melt Analysis -- The Use of Acoustic Mist Ionization Mass Spectrometry for High-throughput Screening -- Ligand Discovery -- High Through-put Binding: Fluorescence Polarization (Anisotropy) -- Fragment Screening by NMR -- A Quick Primer in Fluorescence-based Equilibrium and Pre-Steady State Methods for Determining Protein-Nucleotide Affinities -- Measurement of Nucleotide Hydrolysis using Fluorescent Biosensors for Phosphate -- Gel-based Analysis of Protein-Nucleic Acid Interactions -- Biophysical Studies of the Binding of Viral RNA with the 80S Ribos…

Subjects

Laboratory Manual Laboratory manuals Ligand binding (Biochemistry) Ligand binding (Biochemistry) -- Labora… Ligands Ligands -- Laboratory manuals Ligands -- Manuels de laboratoire Ligands (Biochimie) -- Manuels de labor… Manuels de laboratoire Protein Binding Protein binding -- Laboratory manuals Protéines -- Manuels de laboratoire Protéines -- Manuels de laboratoire. --… Proteins Proteins -- Laboratory manuals