Proteostasis: methods and protocols

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This volume highlights the role of proteostasis in human health and associated disease model systems, reflecting its rising importance which has led to the development of new technologies to obtain insight into underling protein mechanistic events. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips o…

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Title Statements

Full title :Proteostasis : methods and protocols / edited by Rune Matthiesen.

Summary

Edition Statements

Publication Date:©2016

Publication Statements

Publisher(s):Humana Press, Springer
Place of Publication:New York, NY
Language:eng
Publication Date:©2016

The Physical Object

Description:1 online resource (xvi, 488 pages) : : illustrations (some color)

Edition Identifiers

ISBN:9781493937561, 1493937561, 1493937545, 9781493937547
oclc:ocn958424467
LC Classification:QP551
Dewey Decimal:572/.6

UPS Activation in the Battle against Aging and Aggregation-Related Diseases: An Extended Review -- Review And Literature Mining On Proteostasis Factors' And Cancer -- Combining Zebrafish and Mouse Models to Test the Function of Deubiquitinating Enzyme (Dubs) Genes in Development: Role Of Usp45 in the Retina -- Immunodepletion and Immunopurification as Approaches for CSN Research -- Studying Protein Ubiquitylation in Yeast -- Strategies to Detect Endogenous Ubiquitination of a Target Mammalian Protein -- In Vitro Ubiquitination -- Self-Ubiquitination, Chain Formation, and Substrate Ubiquitination Assays -- Isolation of the Ubiquitin-Proteome From Tumour Cell Lines and Primary Cells Using Tubes -- Tubes-Mass Spectrometry for Identification and Analysis of the Ubiquitin-Proteome -- Isolation of Ubiquitinated Proteins to High Purity from In Vivo Samples -- Method for the Purification of Endogenous Unanchored Polyubiquitin Chains -- Fluorescent Tools for In Vivo Studies on the Ubiquitin-Proteasome System -- Bimolecular Fluorescence Complementation to Assay the Interactions of Ubiquitylation Enzymes in Living Yeast Cells -- Monitoring Ubiquitin-Coated Bacteria Via Confocal Microscopy --…

Subjects

Laboratory manuals protein Protein Folding Protein folding -- Laboratory manuals Protéines Protéines -- Manuels de laboratoire Protéines -- Manuels de laboratoire. --… Proteins Proteins -- Laboratory manuals Proteostasis Deficiencies SCIENCE -- Life Sciences