Title Statements
- Full title :Quantitative imaging in cell biology / edited by Jennifer C. Waters, Torsten Wittmann.
Edition Statements
- Publication Date:2014.
- Edition:First edition
Publication Statements
- Publisher(s):Elsevier/AP
- Place of Publication:Amsterdam
- Language:eng
- Publication Date:2014.
The Physical Object
- Description:xx, 568 pages, 20 unnumbered pages of plates : : illustrations (some color) ; : 24 cm
Edition Identifiers
- ISBN:9780124201385, 0124201385
- oclc:ocn885147752
- LC Classification:R857.O6
- Dewey Decimal:570.28/2
Table of Contents
Machine generated contents note: ch. 1 Concepts in Quantitative Fluorescence Microscopy / Torsten Wittmann -- 1.1. Accurate and Precise Quantitation -- 1.2. Signal, Background, and Noise -- 1.3. Optical Resolution: The Point Spread Function -- 1.4. Choice of Imaging Modality -- 1.5. Sampling: Spatial and Temporal -- 1.6. Postacquisition Corrections -- 1.7. Making Compromises -- 1.8.Communicating Your Results -- Acknowledgment -- References -- ch. 2 Practical Considerations of Objective Lenses for Application in Cell Biology / Michael W. Davidson -- Introduction -- 2.1. Optical Aberrations -- 2.2. Types of Objective Lenses -- 2.3. Objective Lens Nomenclature -- 2.4. Optical Transmission and Image Intensity -- 2.5. Coverslips, Immersion Media, and Induced Aberration -- 2.6. Considerations for Specialized Techniques -- 2.7. Care and Cleaning of Optics -- Conclusions -- References -- ch. 3 Assessing Camera Performance for Quantitative Microscopy / Jennifer C. Waters.
Contents note continued: 3.1. Introduction to Digital Cameras for Quantitative Fluorescence Microscopy -- 3.2. Camera Parameters -- 3.3. Testing Camera Performance: The Photon Transfer Curve -- References -- ch. 4 A Prac…
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