The ESCRT complexes: methods and protocols

by Culetto, Emmanuel Legouis, Renaud

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Title Statements

Full title :The ESCRT complexes : methods and protocols / editors, Emmanuel Culetto and Renaud Legouis.

Edition Statements

Publication Date:2019

Publication Statements

Publisher(s):Humana Press
Place of Publication:New York, NY
Language:eng
Publication Date:2019

Notes

1. Analysis of the Archaeal ESCRT Apparatus Rachel Y. Samson, Iain G. Duggin, and Stephen D. Bell 2. Genetic and Cell Biology Methods to Study ESCRTs in Drosophila melanogaster Marco Gualtieri and Thomas Vaccari 3. Functional Analysis of ESCRT-Positive Extracellular Vesicles in the Drosophila Wing Imaginal Disc Tamas Matusek, Pascal Thérond, and Maximilian Fürthauer 4. Subcellular Localization of ESCRT-II in the Nematode C. elegans by Correlative-Light Electron Microscopy Céline Largeau, Emmanuel Culetto, and Renaud Legouis 5. Proximity Ligation Assay (PLA) to Determine the Endosomal Localization of ESCRT Subunit in Virus-Infected Cells Binod Kumar, Mohanan Valiya Veettil, Arunava Roy, and Bala Chandran 6. Immuno-Localization of ESCRT Proteins in Virus-Infected Cells by Fluorescence and Electron Microscopy Keisuke Tabata, Atsuki Nara, Hiroko Omori, and Eiji Morita 7. Single Cell Fluorescence Ratio Image Analysis for Studying ESCRT Function in Receptor Trafficking Jalal M. Kazan, Gergely L. Lukacs, Pirjo M. Apaja, and Arnim Pause 8. Genetic and Biochemical Analyses of Yeast ESCRT Sudeep Banjade, Shaogeng Tang, and Scott D. Emr 9. Live Imaging of ESCRT Proteins in Microfluidically-Isolated Hippocampal Axons Veronica Birdsall, Jose C. Martinez, Lisa Randolph, Ulrich Hengst, and Clarissa L. Waites 10. Studying the Spatial Organization of ESCRTs in Cytokinetic Abscission Using the High Resolution Imaging Techniques SIM and Cryo-SXT Shai Adar-Levor, Inna Goliand, Michael Elbaum, and Natalie Elia 11. Three Dimensional Surface Rendering of ESCRT Proteins Microscopy Data Using UCSF Chimera Software Romain Le Bars, Michele W. Bianchi, and Christophe Lefebvre 12. Transient Expression of ESCRT-Components in Arabidopsis Root Cell Suspension Culture-Derived Protoplasts Marie-Kristin Nagel, Karin Vogel, and Erika Isono 13. Crystallization and Biophysical Approaches for Studying the Interactions between the Vps4-MIT Domain and ESCRT-III Proteins Takayuki Obita, Rieko Kojima, and Mineyuki Mizuguchi 14. Biochemical Approaches to Studying C. elegans ESCRT Function In Vitro Samuel Block, Amber L. Schuh, and Anjon Audhya 15. Purification of Recombinant ESCRT-III Proteins and Their Use in Atomic Force Microscopy and In Vitro Binding and Phosphorylation Assays Luisa Capalbo, Ioanna Mela, Maria Alba Abad, A. Arockia Jeyaprakash, J. Michael Edwardson, and Pier Paolo D'Avino 16. Assessment of ESCRT Protein CHMP5 Activity on Client Protein Ubiquitination by Immunoprecipitation and Western Blotting Francheska Son, Katharine Umphred-Wilson, Jae-Hyuck Shim, and Stanley Adoro 17. Purification of Plant ESCRT Proteins for Polyclonal Antibody Production Julio Paez-Valencia and Marisa S. Otegui 18. Genetic and Cytological Methods to Study ESCRT Cell Cycle Function in Fission Yeast Imane M. Rezig, Shaun K. Bremner, Musab S. Bhutta, Ian P. Salt, Gwyn W. Gould, and Christopher J. McInerny 19. ESCRT Mutant Analysis and Imaging of ESCRT Components in the Model Fungus Ustilago maydis Carl Haag, Thomas Klein, and Michael Feldbrügge 20. Genetic Suppressor Screen Using an Inducible FREE1-RNAi Line to Detect ESCRT Genetic Interactors in Arabidopsis thaliana Qiong Zhao, Ying Zhu, Wenhan Cao, Jinbo Shen, Yong Cui, Shuxian Huang, and Liwen Jiang 21. Screening of Interactions with the ESCRT Machinery by a Gaussia princeps Split Luciferase-Based Complementation Assay Rina Barouch-Bentov, Yves Jacob, and Shirit Einav 22. RNA Interference-Mediated Inhibition of ESCRT in Mammalian Cells Katherine Bowers.