Rhinoviruses: methods and protocols
by Ghildyal, Reena Jans, David A.
"Rhinoviruses: Methods and Protocols highlights the numerous molecular, cellular and in vivo tools now available to conduct human rhinovirus (HRV) research in an effort to increase understanding of the clinical disease caused by HRVs as well as the functions of its individual proteins and its replication. Human rhinoviruses are the major cause of common colds as well as being more recently recognized as the major viral cause of asthma and chronic obstructive pulmonary disease (COPD) exacerbatio…
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- Full title :Rhinoviruses : methods and protocols / edited by David A. Jans, Reena Ghildyal.
Summary
"Rhinoviruses: Methods and Protocols highlights the numerous molecular, cellular and in vivo tools now available to conduct human rhinovirus (HRV) research in an effort to increase understanding of the clinical disease caused by HRVs as well as the functions of its individual proteins and its replication. Human rhinoviruses are the major cause of common colds as well as being more recently recognized as the major viral cause of asthma and chronic obstructive pulmonary disease (COPD) exacerbations. The huge advancements in technical applications of biophysics and improvements in high-end imaging techniques have also had implications for HRV research among other infectious diseases. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Rhinoviruses: Methods and Protocols seeks to serve both professionals and novices with the latest technical advances in HRV research to ultimately enable the development of therapeutic approaches to combat HRV, and most importantly, its pathogenic effects in asthma exacerbation."--Page 4 of cover.
Read More ↓Edition Statements
- Publication Date:2015
Publication Statements
- Publisher(s):Humana Press
- Place of Publication:New York
- Language:eng
- Publication Date:2015
The Physical Object
- Description:xii, 190 pages : : illustrations (some colour) ; : 27 cm
Edition Identifiers
- ISBN:9781493915705, 1493915703
- oclc:ocn882189083
- LC Classification:QR410.2.R45
- Dewey Decimal:616.9/101
Table of Contents
Classification and evolution of human rhinoviruses / Ann C. Palmenberg and James E. Gern -- Nested-RT-PCR and multiplex RT-PCR for diagnosis of rhinovirus infection in clinical samples / Xuelian Yu and Reena Ghildyal -- Molecular identification and quantification of human rhinoviruses in respiratory samples / Wai-Ming Lee [and others] -- Molecular genotyping of human rhinovirus by using PCR and sanger sequencing / Wei Wang [and others] -- Growth of human rhinovirus in H1-HeLa cell suspension culture and purification of virions / Wai-Ming Lee [and others] -- Propagation of rhinovirus-C strains in human airway epithelial cells differentiated at air-liquid interface / Shamaila Ashraf, Rebecca Brockman-Schneider, and James E. Gern -- Infectivity assays of human rhinovirus-A and -B serotypes / Wai-Ming Lee [and others] -- Application of FCS in studies of rhinovirus receptor binding and uncoating / Shushan Harutyunyan [and others] -- Capillary electrophoresis, gas-phase electrophoretic mobility molecular analysis, and electron microscopy : effective tools for quality assessment and basic rhinovirus research / Victor U. Weiss [and others] -- Proteases of human rhinovirus : role in infect…
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