Riboswitch discovery, structure and function

Riboswitch discovery, structure and function

by Burke-Aguero, Donald H.

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Title Statements

  • Full title :Riboswitch discovery, structure and function.

Edition Statements

  • Publication Date:2014.

Publication Statements

The Physical Object

  • Description:XXIII, 546 str. : : ilustr. ; : 25 cm +

Edition Identifiers

  • ISBN:9780128011225, 012801122X
  • oclc:ocn911550565

Table of Contents

Riboswitch discovery by combining RNA-seq and genome-wide indentification of transcriptional start sites -- Discoverying human RNA aptamers by structure-based bioinformatics and genome-based in vitro selection -- Affinity purification of in vitro transcribed RNA with homogenous ends using a 3'-ARiBo tag -- Deoxyribozyme-mediated ligation for incorporating EPR spin labels and reporter groups into RNA -- A flexible, scalable method for preparation of homogenous aminoacylated tRNAs -- Synthesis of a biotinylated photocleavable nucleotide monophosphate for the preparation of natively folded RNAs -- Chemo-enzymatic synthesis of selectively 13C/15N-labeled RNA for NMR structural and dynamics studies -- SHAPE analysis of small RNAs and riboswitches -- Experimental approaches for measuring pKa's in RNA and DNA -- Crystallographic analysis of TPP riboswitch binding by small-molecule ligands discovered through fragment-based drug discovery approaches -- Methods for using new conceptual tools and parameters to assess RNA structure by small-angle X-ray scattering -- Use of 19F NMR methods to probe conformational heterogeneity and dynamics of exchange in functional RNA molecules -- Site-direct…

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Subjects

Complexes récepteur-ligand molekularna genetika Petits ARN interférents Receptor-ligand complexes Riborégulateurs Riboswitches Small interfering RNA

Writers

Burke-Aguero, Donald H.

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