RNA-protein complexes and interactions

RNA-protein complexes and interactions: methods and protocols

by Lin, Ren-Jang

Type Book
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This detailed volume explores the continuing techniques of studying RNA-protein complexes and interactions as research in these areas expand. After an introductory chapter, it continues with ways to purify RNA-protein complexes assembled in cells or in isolated cellular extracts, methods for measuring various biochemical activities of RNA-interacting proteins or ribonucleoproteins, biochemical methods for measuring direct RNA-protein contact, as well as various new or innovative methods pertine…

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Title Statements

  • Full title :RNA-protein complexes and interactions : methods and protocols / edited by Ren-Jang Lin.

Summary

This detailed volume explores the continuing techniques of studying RNA-protein complexes and interactions as research in these areas expand. After an introductory chapter, it continues with ways to purify RNA-protein complexes assembled in cells or in isolated cellular extracts, methods for measuring various biochemical activities of RNA-interacting proteins or ribonucleoproteins, biochemical methods for measuring direct RNA-protein contact, as well as various new or innovative methods pertinent to the subject.

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Edition Statements

  • Publication Date:2016

Publication Statements

  • Publisher(s):Humana Press
  • Place of Publication:New York
  • Language:eng
  • Publication Date:2016

The Physical Object

  • Description:xiii, 288 pages : : illustrations (some color) ; : 26 cm

Edition Identifiers

  • ISBN:9781493935895, 1493935895
  • oclc:ocn932095861
  • LC Classification:QP623.8.P75
  • Dewey Decimal:572.8/8

Table of Contents

Characterization of RNA-Protein Interactions : Lessons from Two RNA-Binding Proteins, SRSF1 and SRSF2 -- Identification of mRNA-Interacting Factors by MS2-TRAP (MS2-Tagged RNA Affinity Purification) -- Biotin-Streptavidin Affinity Purification of RNA-Protein Complexes Assembled In Vitro -- Detecting RNA-Protein Interaction Using End-Labeled Biotinylated RNA Oligonucleotides and Immunoblotting -- Purification of RNA-Protein Splicing Complexes Using a Tagged Protein from In Vitro Splicing Reaction Mixture -- Loading of Argonaute Protein with Small Duplex RNA in Cellular Extracts -- In Vitro Analysis of Ribonucleoprotein Complex Remodeling and Disassembly -- Single-Turnover Kinetics of Methyl Transfer to tRNA by Methyltransferases -- Purification and Functional Reconstitution of Box H/ACA Ribonucleoprotein Particles -- Northwestern Blot Analysis: Detecting RNA-Protein Interaction After Gel Separation of Protein Mixture -- Measuring mRNA Translation by Polysome Profiling -- Genome-Wide Profiling of RNA-Protein Interactions Using CLIP-Seq -- Identification of Endogenous mRNA-Binding Proteins in Yeast Using Crosslinking and PolyA Enrichment -- Ribo-Proteomics Approach to Profile RNA-Pro…

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Subjects

Interactions ARN-protéines -- Manuels d… Laboratory Manual Laboratory manuals Manuels de laboratoire Protéines de liaison à l'ARN RNA BINDING PROTEINS RNA-protein interactions RNA-protein interactions -- Laboratory …

Writers

Lin, Ren-Jang

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