RNA spectroscopy: methods and protocols

by Arluison, Véronique Wien, Frank

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Title Statements

Full title :RNA spectroscopy : methods and protocols / edited Véronique Arluison, Frank Wien.

Edition Statements

Publication Date:[2020].

Publication Statements

Publisher(s):Humana Press
Place of Publication:New York, NY
Language:eng
Publication Date:[2020].

Notes

Preface...Table of Contents...Contributing Authors... 1. Encapsulation of Fluorescently Labelled RNAs into Surface-Tethered Vesicles for Single-Molecule FRET Studies in TIRF MicroscopySusann Zelger-Paulus, Mélodie C.A.S. Hadzic, Roland K.O. Sigel, and Richard Börner 2. Preparation of SNAPf-Beads for Colocalization Single-Molecule Spectroscopy (CoSMoS) of RNA-Protein ComplexesReka A. Haraszti and Joerg E. Braun 3. Comparative Colocalization Single-Molecule Spectroscopy (CoSMoS) with Multiple RNA SpeciesReka A. Haraszti and Joerg E. Braun 4. Dynamic Light Scattering Analysis on RNA Associated to ProteinsSerena Bernacchi 5. Bioinformatic Application of Fluorescence-Based In Vivo RNA Regional Accessibility Data to Identify Novel sRNA TargetsEmily K. Bowman, Mia K. Mihailovic, Bridget Li, and Lydia M. Contreras 6. Surface Plasmon Resonance for Investigating Molecular Interactions with RNACarmelo Di Primo 7. RNA folding and Unfolding Under Force: Single-Molecule Experiments and their AnalysisLaurent Geffroy, Thierry Bizebard, and Ulrich Bockelmann 8. Mapping of Post-Transcriptional tRNA Modifications by Two-Dimensional Gel Electrophoresis Mass SpectrometryLaura Antoine and Philippe Wolff 9. Native Electrospray Ionization Mass Spectrometry of RNA-Ligand ComplexesPhilippe Wolff and Eric Ennifar 10. Application of FTIR Spectroscopy to Analyse RNA StructureFredéric Geinguenaud, Valeria Militello, and Véronique Arluison 11. Application of Synchrotron Radiation Circular Dichroism for RNA Structural AnalysisEtienne Le Brun, Véronique Arluison, and Frank Wien 12. Application of NIR Raman Spectroscopy to Probe the Flexibility of RNA StructureHui Bon Hoa Gaston 13. Small Angle Neutron Scattering of RNA-Protein ComplexesAudrone Lapinaite, Teresa Carlomagno, and Frank Gabel 14. Structural Analysis of RNA by Small-Angle X-Ray ScatteringAnne Théobald-Dietrich, Raphaël de Wijn, Kévin Rollet, Alexandra Bluhm, Joëlle Rudinger-Thirion, Caroline Paulus, Bernard Lorber, Aurélien Thureau, Magali Frugier, and Claude Sauter 15. Site-Specific Spin-Labeling of RNA for NMR and EPR Structural StudiesBertrand Vileno and Isabelle Lebars 16. Analysis of the HIV-1 Genomic RNA Dimerization Initiation Site Binding to Aminoglycoside Antibiotics using Isothermal TitrationSerena Bernacchi and Eric Ennifar 17. Use of Fluorescent Nucleotides to Map RNA-Binding Sites on Protein SurfaceV. Balobanov, N. Lekontseva, A. Mikhaylina, and A. Nikulin 18. Fluorescent Oligonucleotide Probes for the Quantification of RNA by Real-Time qPCRFlorent Busi 19. Probing RNA-Protein Interactions and RNA Compaction by Sedimentation Velocity Analytical UltracentrifugationSomdeb Mitra and Borries Demeler 20. RNA Nanostructures Molecular ImagingOlivier Pietrement, Véronique Arluison, and Christophe Lavelle 21. Grad-CryoEM: Tool to Isolate Translation Initiation Complexes from Rabbit Reticulocyte Lysate Suitable for Structural StudiesJavier Rol-Moreno, Lauriane Kuhn, Stefano Marzi, and Angelita Simonetti 22. Application of NMR Spectroscopy to Determine Small RNA StructurePengzhi Wu, Xiaodan Liu, Qingguo Gong, Yunyu Shi, and Jihui Wu.

The Physical Object

Description:1 online resource (xi, 356 pages) : : illustrations (some color).

Edition Identifiers

ISBN:9781071602782, 1071602780, 1071602772, 9781071602775
oclc:on1308639898
LC Classification:QP623
Dewey Decimal:572.8/8

Subjects

ARN -- Manuels de laboratoire Laboratory Manual Laboratory manuals Manuels de laboratoire Medical genetics RNA RNA -- Laboratory manuals RNA Folding -- physiology SCIENCE -- Spectroscopy & Spectrum Anal… Spectrum Analysis -- methods Spectrum analysis, spectrochemistry, ma…