Surface plasmon resonance
SPR is real-time, label-free measurements of binding kinetics and affinity. This has distinct advantage over radioactive or fluorescent labeling methods, in terms of 1) ligand-analyte binding kinetics, that can be probed without the costly and time-consuming labeling process that may interfere with molecular binding interactions; 2) binding rates and affinity can be measured directly and 3) low affinity interactions in high protein concentrations for can be characterized with less reagent consu…
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- Full title :Surface plasmon resonance / Pranveer Singh.
Summary
SPR is real-time, label-free measurements of binding kinetics and affinity. This has distinct advantage over radioactive or fluorescent labeling methods, in terms of 1) ligand-analyte binding kinetics, that can be probed without the costly and time-consuming labeling process that may interfere with molecular binding interactions; 2) binding rates and affinity can be measured directly and 3) low affinity interactions in high protein concentrations for can be characterized with less reagent consumption than other equilibrium measurement techniques; 4) Label-free detection of molecular interactio.
Read More ↓Edition Statements
- Publication Date:2014
Publication Statements
- Publisher(s):Novinka
- Place of Publication:New York
- Language:eng
- Publication Date:2014
The Physical Object
- Description:1 online resource.
Edition Identifiers
- ISBN:9781633218666, 163321866X
- oclc:on1182019940
- LC Classification:QC176.8.P55
- Dewey Decimal:530.4/16
Table of Contents
""SURFACE PLASMON RESONANCE""; ""SURFACE PLASMON RESONANCE""; ""Library of Congress Cataloging-in-Publication Data""; ""CONTENTS""; ""PREFACE""; ""Chapter 1: INTRODUCTION""; ""OVERVIEW OF SURFACE PLASMON RESONANCE (SPR)""; ""1.1. PHYSICAL BASIS OF SURFACE PLASMON RESONANCE""; ""1.2. STEP BY STEP WORKING OF SPR""; ""Chapter 2: PRINCIPLES AND MECHANISM BEHIND SPR""; ""2.1. PHYSICAL BASIS OF SPR""; ""2.2. LOCALIZED SURFACE PLASMON RESONANCE (LSPR)""; ""2.3. SPR EMISSION""; ""Chapter 3: INSTRUMENTS BASED ON SPR PHENOMENA""; ""3.1. HISTORICAL OVERVIEW""
""3.2. PRINCIPLES AND MECHANISM BEHIND WORKING OF SPR BASED INSTRUMENTS""""Chapter 4: APPLICATIONS""; ""4.1. OPTICAL SENSOR BASED ON SPR OPERATING IN THE MID-INFRARED RANGE""; ""4.2. ADVANTAGES OF SPR""; ""4.3. BINDING KINETICS OF A MODEL ANTIBODY-ANTIGENSYSTEM, HSA BINDING TO ANTI-HSA IGG.ANTI-HSA IS BIOTINYLATED WITH APPROXIMATELY6 BIOTIN GROUPS AND CAPTURED ONA PLANAR NEUTR-AVIDIN SENSOR SLIDE""; ""4.4. SPR BINDING EXPERIMENT BETWEEN CAII ANDAN INHIBITOR, 4-CRBOXYBENZENESULFONAMIDE(4-CBS); A SMALL MOLECULE WITH A MOLECULARWEIGHT OF 201 DA""
""4.5. THERMODYNAMIC INVESTIGATION OFAN ENZYME-INHIBITOR PAIR""""4.6. SMALL VOLUME INJECTION…
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