Synthetic gene circuits: methods and protocols

by Menolascina, Filippo

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Title Statements

Full title :Synthetic gene circuits : methods and protocols / edited by Filippo Menolascina,....

Summary

AThis volume provides clear and direct protocols to implement automated Design-Build-Test-Learn (DBTL) into synthetic biology research. Chapters detail techniques to model and simulate biological systems, redesign biological systems, setting up of an automated biolaboratory, step-by-step guide on how to perform computer aided design, RNA sequencing, microfluidics -using bacterial cell free extracts, live mammalian cells, computational and experimental procedures, metabolic burden, computational techniques to predict such burden from models, and how DNA parts can be engineered in mammalian cells to sense, and respond to, and intracellular signals in general. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Synthetic Gene Circuits: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Edition Statements

Publication Date:copyright 2021

Publication Statements

Publisher(s):Humana Press
Place of Publication:New York
Language:eng
Publication Date:copyright 2021

Notes

Publié aussi sous forme numérique avec l'e-ISBN 978-1-0716-1032-9.

The Physical Object

Description:1 vol. (x-353 p.) : : ill. en noir et en coul. ; : 26 cm.

Edition Identifiers

ISBN:9781071610312 (rel), 1071610317 (rel)
oclc:on1293866003

Subjects

Biologie de synthèse Biologie synthétique -- méthodes Génie tissulaire Laboratory manuals Réseaux de régulation génique Synthetic Biology Synthetic biology -- Laboratory manuals