The ELISA guidebook

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This text presents the fundamental aspects of the heterogeneous enzyme linked immunosorbent assay (ELISA) used in biomedical and agricultural research. This book gives numerous examples of the use of ELISA with detailed instructions so that investigators can understand, develop, and apply ELISA methodology in day-to-day basic and clinical research.

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Title Statements

Full title :The ELISA guidebook / by John R. Crowther.

Summary

Edition Statements

Publication Date:©2009.
Edition:2nd ed

Publication Statements

Publisher(s):Humana Press
Place of Publication:New York, NY
Language:eng
Publication Date:©2009.

Notes

Previous edition: 2001.

The Physical Object

Description:xv, 566 pages : : illustrations ; : 27 cm.

Edition Identifiers

ISBN:9781603272537, 1603272534, 9781603272544, 1603272542
DOI:10.1007/978-1-60327-254-4.
oclc:ocn258096611
LC Classification:QH506
Dewey Decimal:616.0756

Overview of ELISA in relation to other disciplines -- Systems in ELISA -- Stages in ELISA -- Titration of reagents -- Theoretical considerations -- Practical exercises -- Monoclonal antibodies -- Validation of diagnostic tests for infectious diseases -- Charting methods for internal quality control for competition ELISA -- Charting methods for internal quality control of indirect ELISA -- Ruggedness and robustness of tests: aspects of kit use and validation -- More advanced statistical methods for quality assurance, test validation, and interpretation -- Internal quality control and external quality management of data in practice -- Immunochemical techniques -- Test questions.