The golgi complex: methods and protocols

by Brown, William J., (Professor de biologia cel·lular)

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Title Statements

Full title :The golgi complex : methods and protocols / edited by William J. Brown, Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY, USA.

Summary

"Focusing on new methods and techniques developed to address longstanding questions about the Golgi complex, this volume explores a diverse set of chapters, ranging from live and fixed cell imaging techniques toin vitro biochemical reconstitution systems. Each chapter provides a detailed set of specific instructions, which should enable anyone to successfully complete the assays. Written for the highly successful Methods in Molecular Biology series program, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips from the experts on troubleshooting and avoiding known pitfalls. Practical and detailed, The Golgi Complex: Methods and Protocols will aid both new and established researchers in the field by providing strong hands-on instructions that can be directly applied to their research programs.iv>."--OCLC.

Edition Statements

Publication Date:2016

Publication Statements

Publisher(s):Humana Press, Springer
Place of Publication:New York, NY
Language:eng
Publication Date:2016

The Physical Object

Description:1 online resource (1 recurs en línia (xi, 219 pàgines) :) : : il·lustracions (algunes en color).

Edition Identifiers

ISBN:9781493964635, 1493964631
oclc:ocn994745908

Conté: 4D confocal imaging of yeast organelles -- Imaging the polarized sorting of proteins from the golgi complex in live neurons -- Imaging golgi outposts in fixed and living neurons -- Analysis of Arf1 GTpase-dependent membrane binding and remodeling using the exomer secretory vesicle cargo adaptor -- STEM tomography imaging of hypertrophied golgi stacks in mucilage-secreting cells -- Reconstitution of COPI vesicle and tubule formation -- Reconstitution of Phospholipase A 2 -Dependent Golgi Membrane Tubules -- Proteomic characterization of golgi membranes enriched from Arabidopsis suspension cell cultures -- High-content analysis of the golgi complex by correlative screening microscopy -- Activity detection of GalNAc transferases by protein-based fluorescence sensors in vivo -- In situ proximity ligation assay (PLA) analysis of protein complexes formed between golgi-resident, glycosylation-related transporters and transferases in adherent mammalian cell cultures -- Creating knockouts of conserved oligomeric golgi complex subunits using CRISPR-mediated gene editing paired with a selection strategy based on glycosylation defects associated with impaired COG complex function -- Re…