The tumor microenvironment: methods and protocols

by Beauchemin, Nicole Ursini-Siegel, Josie

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Title Statements

Full title :The tumor microenvironment : methods and protocols / edited by Josie Ursini-Siegel, Nicole Beauchemin.

Summary

This book covers core and emerging in vitro and in vivo protocols used to study how various components of the tumor microenvironment are established and subsequently interact with tumor cells to facilitate carcinogenesis. In addition, the book examines research topics including cellular and molecular biology approaches, in vivo genetic approaches, various omics -based strategies, therapeutic strategies to target the microenvironment, and, finally, advanced techniques in the fields of tissue engineering and nanotechnology. Written and validated in the laboratories of a number of trusted collaborating authors for the highly successful Methods in Molecular Biology series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and authoritative, The Tumor Microenvironment: Methods and Protocols constitutes a compendium of techniques now available to a broad audience, including basic and clinician scientists, systems biologists, and biological engineers."

Edition Statements

Publication Date:2016

Publication Statements

Publisher(s):Humana Press
Place of Publication:New York
Language:eng
Publication Date:2016

The Physical Object

Description:xii, 356 pages : : illustrations (some color) ; : 26 cm

Edition Identifiers

ISBN:9781493937998, 1493937995
oclc:ocn945356774
LC Classification:RC254.5
Dewey Decimal:616.99/4

Methods of Immunohistochemistry and Immunofluorescence: Converting Invisible to Visible -- Laser Capture Microdissection as a Tool to Study Tumor Stroma -- Quantitative Analysis of Human Cancer Cell Extravasation Using Intravital Imaging -- Studies on the Tumor Vasculature and Coagulant Microenvironment -- A Microfluidic Method to Mimic Luminal Structures in the Tumor Microenvironment -- Measuring Vascular Permeability In Vivo -- Hydroxylation-Dependent Interaction of Substrates to the Von Hippel-Lindau Tumor Suppressor Protein (VHL) -- Analyzing the Tumor Microenvironment by Flow Cytometry -- Detecting Secreted Analytes from Immune Cells: An Overview of Technologies -- Purification of Immune Cell Populations from Freshly Isolated Murine Tumors and Organs by Consecutive Magnetic Cell Sorting and Multi-Parameter Flow Cytometry-Based Sorting -- Viral Engineering of Chimeric Antigen Receptor Expression on Murine and Human T Lymphocytes -- Methods to Evaluate the Anti-Tumor Activity of Immune Checkpoint Inhibitors in Preclinical Studies -- Isolation and Characterization of Low Vs. High-Density Neutrophils in Cancer -- Analysis of Extracellular Vesicles in the Tumor Microenvironment --…

Subjects

Carcinogenesis -- genetics Cytopathologie Laboratory Manual Laboratory manuals Manuels de laboratoire neoplasms Pathology, Cellular Tumeurs Tumeurs -- Cytopathologie Tumor Microenvironment -- genetics Tumors Tumors -- Cytopathology