Title Statements
- Full title :Understanding light microscopy / by Jeremy Sanderson.
Edition Statements
- Publication Date:2018.
- Edition:First edition
Publication Statements
- Publisher(s):John Wiley & Sons
- Place of Publication:Hoboken, NJ
- Language:eng
- Publication Date:2018.
The Physical Object
- Description:1 online resource.
Edition Identifiers
- ISBN:9781118696743, 1118696743, 9781118696811, 1118696816
- oclc:on1196821445
- LC Classification:QH205.2
- Dewey Decimal:570.28/2
Table of Contents
Our eyes and the microscope -- Light -- Basic microscope optics -- Microscope design -- Ergonomics -- Optical aberrations of the microscope -- The microscope objective -- Condensers & eyepieces -- Illumination in the microscope -- Diffraction and image formation in microscopy -- Contrast generation & enhancement -- Reflected-light microscopy -- Polarised-light microscopy : part 1, theory -- Polarised-light microscopy : part 2, applied -- Fluorescence microscopy -- Fluorophores & fluorescent proteins -- Optical sectioning & confocal microscopy -- Operating the confocal microscope -- Light-sheet microscopy -- Bleed-through & spectral unmixing -- Deconvolution -- Multi-photon microscopy -- Total internal reflection fluorescence microscopy -- Frap and fret -- Colocalisation -- Super-resolution fluorescence microscopy -- Choosing a microscope platform and core imaging facilities -- Biological specimen preparation -- Materials specimen preparation -- Recording the image : part 1, theory -- Recording the image : part 2, applied.
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