Enzyme-linked immunospot assay (ELISPOT) has been known for some time as a unique state-of-the-art technique for studying the cytokine-secreting activity of immune system cells, and it appears to be one of the fast growing applications in biomedical research, becoming an indispe…
Research into the field of stem cell biology has developed exponentially over recent years, and is beginning to offer significant promise for unravelling the molecular basis of a multitude of disease states. Importantly, in addition to offering the opportunity to delve deeply in…
Recent outbreaks of swine influenza and avian influenza, along with the remaining and in some cases expanding threats from HIV, dengue virus, and the viruses causing hepatitis, have reinforced the need for rapid, accurate and cost-effective diagnosis of viral disease. Diagnostic…
by Deb, Sumitra, Deb, Swati Palit
fromMethods in molecular biology, methods and protocols
The second edition of p53 Protocols expands upon the first edition with progress in p53 research since the publication of the first edition. In p53 Protocols, Second Edition expert researchers in the field detail many of the methods that address the challenging questions of the …
by Brooks, Susan A., Harris, Adrian
fromMethods in molecular medicine
Many new technologies involving large-scale analysis of gene expression, novel treatments, and emergent combined therapy approaches have opened fresh possibilities for understanding and effectively managing breast cancer. In Breast Cancer Research Protocols, active researchers a…
Biolistic transfection represents a direct physical gene transfer approach in which nucleic acids are precipitated on biologically inert high-density microparticles (usually gold or tungsten) and delivered directly through cell walls and/or membranes into the nucleus of target c…
Scientists investigating germ cells have, over the past 15 years, originated discoveries and innovations that give us valuable insights into the mechanisms that regulate not just stem cell function, but human development in its widest sense. With contributions from some of the l…
In Protein Dynamics: Methods and Protocols, expert researchers in the field detail both experimental and computational methods to interrogate molecular level fluctuations. Chapters detail best-practice recipes covering both experimental and computational techniques, reflecting m…
Transcranial Magnetic Stimulation seeks to enable new and existing practitioners to learn and follow established TMS protocols. The individual chapters in this detailed volume describe methods for single and multiple pulse TMS as well as methods for TMS coupled with associated t…
Biomimetics and Stem Cells: Methods and Protocols collects a series of approaches to demonstrate the role and value of biomimetics for the better understanding of stem cell behavior and the acceleration of their application in regenerative medicine. Recent advances in tissue eng…
This volume details protocols on detection and analysis of fusion events in various cell types in vitro and in vivo. Additional, protocols aim to facilitate the future highly relevant process of engineering cells for specific purposes, such as generation of transgenic embryos an…
Protein Design: Method and Applications, Second Edition expands upon the previous edition with current, detailed ideas on how to approach a potential protein design project. With new chapters on metals as structure-forming elements and functional sites, the design and characteri…
"This book covers the concepts of molecular medicine and personalized medicine. Subsequent chapters cover the topics of genomics, transcriptomics, epigenomics, and proteomics, as the tools of molecular pathology and foundations of molecular medicine. These chapters are followed …
This unique book presents an up-to-date discussion of clinical disorders of the pituitary gland in children with specific emphasis on state-of-the-art diagnostic and treatment modalities, highlighting the newest scientific advances in genomics and molecular biology that clinicia…
This unique book presents an up-to-date discussion of clinical disorders of the pituitary gland in children with specific emphasis on state-of-the-art diagnostic and treatment modalities, highlighting the newest scientific advances in genomics and molecular biology that clinicia…
This book is a comprehensive and richly-illustrated guide to cardiac CT, its current state, applications, and future directions. While the first edition of this text focused on what was then a novel instrument looking for application, this edition comes at a time where a wealth …
This comprehensive volume examines the role of vitamin E as an essential micronutrient in human health and its extensive clinical benefits. Sections cover a wide breadth of topics, including vitamin E intake and recommended daily allowance, understanding the biological activitie…
This comprehensive volume examines the role of vitamin E as an essential micronutrient in human health and its extensive clinical benefits. Sections cover a wide breadth of topics, including vitamin E intake and recommended daily allowance, understanding the biological activitie…
This book reviews recent knowledge of the role of stem cells in the gastrointestinal system. It covers extensive topics for each organ, including the pancreas, esophagus, liver, and colon, while also discussing the contributions of stem cells to therapeutic approaches toward gas…
