A polygalacturonase-inhibiting protein (PGIP) was purified from tomato fruit and stem tissue and was subsequently characterized. PGIP was found in stem, leaf and fruit tissue of tomato but not in root tissue. The PGIP was purified by a combination of ion-exchange, gel filtration…
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Species of the genus Aspergillus serve as model systems for transformation and gene expression studies in filamentous fungi. In this investigation, the plasmid vectors pP01/2α, with replicative potential in the basidiomycete Pleurotus ostreatus, were further analyzed. They were …
Weak organic acids and/or their salts were tested as soil washing or flushing agents for the ex- or in-situ remediation of soils polluted by heavy metals. Three soils naturally with heavy metals were used for the tea. The three soils were characterized as a clay loam, loam and s…
Filamentous fungi are used in the production of both homologous and heterologous proteins due to their desirable growth characteristics. However, the product concentrations of heterologous proteins are usually much lower than those of homologous proteins. Apart from the difficul…
One of the most important aspects of food stability to be monitored is the potential to support microbial growth and/or toxin production. Many researches have shown that water activity (a {w}) cannot adequately account for the observed stability behavior of many food systems. We…
Among the various fungal strains screened for citric acid production, Aspergillus niger is known to produce considerable amounts of citric acid and other organic acids when cultivated in carbohydrate-rich medium in solid substrate fermentation (SSF). Since A. niger on a solid su…
Cellulose is the major component of wood. Cellulases degrade the cellulose polymer by cleaving the glycosidic linkage between glucose monomers. Cellulases can be either useful, by producing energy from cellulose degradation, or harmful, by destruction of structural wood. Here, w…
In this study, the potential of fungi Aspergillus niger to remove cadmium, copper, lead and nickel ions from an aqueous solution was evaluated. Several pretreatment alternatives were investigated for enhancing metal removal capacity of live A. niger biomass. Cadmium, copper and …
The screening, biomass production of lipase-producing microorganisms from several sources, as well as the purification, characterization and utilization of the enzymatic extracts for the interesterification of butter fat were investigated. Pseudomonas fragi CRDA 323 and Aspergil…
A 190 kDa β-glucosidase purified from commercial Aspergillus niger cellulase was shown to be a dimer and to be N-glycosylated (comprised mainly of mannose). It had unique pH dependent kinetics at high substrate concentrations. This was explained by the finding that the β-glucosi…
Among industrial wastewaters, dye wastewater from textile and dyestuff industries is one of the most difficult to treat because of its synthetic origin and complex aromatic molecular structure of dyes. Biosorption using fungi is becoming a promising alternative to replace or sup…
Five forms of endoglucanase were isolated from culture fluid of V. volvacea grown on crystalline cellulose following ion-exchange, gel filtration, hydrophobic interaction (Phenyl-Sepharose) chromatography, and preparative polyacrylamide gel electrophoresis. All had the same mole…
Cellulose and xylan are the two major polysaccharides found in plant fiber and represent a vast feedstock source for microbial ethanol production. To render the cellulose amenable to bioconversion, xylan is removed by a dilute sulfuric acid pretreatment, and at present, not util…
Despite the recent advances in antifungal development, fungi remain a highly destructive threat to human health and compromise food viability. Though chemical fungicides today provide the primary means of postharvest pathogenic fungal control, their continuous use is faced with …
La premiere partie du present document met une emphase sur l'hydrolyse enzymatique des dechets solides des pates et papiers dans le but de produire des sucres reduits. Le traitement a l'aide d'enzymes permet de valoriser des dechets cellulosiques abondants qui deviennent alors u…
The objective of this research was to express a fungal phytase gene in transgenic soybean cells to to study the potential for improving phosphorus utilization in soybean meal. A simple and inexpensive particle inflow gene gun was constructed and bombardment was optimized as assa…
Solid state fermentation of canola meal has been carried out for the reduction of its phytic acid content. Several microorganisms were surveyed for this purpose. The phytic acid content of canola meal was reduced by 26%, 60% and 66% using Saccharomyces cerevisiae, Rhizopus oligo…
Survival and development of Aedes triseriatus, the mosquito vector of La Crosse Encephalitis, were found to be inhibited in the presence of dried leaves of certain populations of the sugar maple Acer saccharum . It is suspected that these effects are due to fungal contaminants c…
The projects described in this dissertation are mainly focused on exploration of marine-derived fungi as a source of new pharmacologically active natural products. Fungi are prolific sources of medically useful secondary metabolites. Currently, fungi from marine habitats are rel…