Escherichia coli synthesizes and secretes the iron-chelating siderophore enterobactin under conditions of iron stress. Six genes for enterobactin biosynthesis, entABCDEF, five genes for the transport of ferrienterobactin, fepABCDG, and the gene for ferrienterobactin hydrolysis, …
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A fermented sausage process that achieves a five-log reduction of Escherichia coli O157:H7 meets current USDA/FSIS requirements. The objectives of this study were: (1) to determine the effects of representative Lebanon bologna fermentation and thermal processing temperatures and…
Many sources of pollution, including agricultural, industrial, and urban sources, can increase a stream or lake's nutrient and bacterial concentrations. In order to improve water quality, these sources must be identified, and microbial source tracking (MST) methods are currently…
The Escherichia coli promoter, pBAD, under the control of the dimeric AraC protein, drives the expression of the AraB, AraA and AraD gene products of the arabinose operon. In this work, I have used the arabinose system to: (1) Study the recognition of overlapping nucleotides by …
Three different fluorescence-based rapid detection assays were developed for the detection of pathogenic bacteria in food. The following research focuses on both the capabilities of each assay, as well as on the development of an idea into a working assay. A solid phase fluoresc…
Avian pathogenic strains of Escherichia coli serotypes O2, O35 and O78 cause air sac infections in turkeys, but the reason for the colonization of these serotypes is unknown. Air sac surface fluid (ASSF) from 8–12 weeks old turkeys was examined to identify proteins that bind sur…
Pasteurellosis has plagued rabbits since time immemorial. The treatment of Pasteurella multocida infection is often frustrating. The goal was to investigate application of a broad spectrum vaccine based on conserved gram-negative bacterial core antigens (GNCA) that would provide…
Archived formalin-fixed intestinal tissues, and frozen intestinal contents collected from 12 PEMS flocks in 1998 were used to determine the prevalence of EPEC in PEMS-affected turkey flocks. EPEC isolates were assessed for pathogenicity and ability to reproduce AE lesions in you…
Pathogenic E. coli infections cause approximately one quarter of disease losses in commercial turkey flocks. A small subgroup of E. coli causes most infections. Epidemiologic studies of this disease have been hindered by a lack of reliable markers to discriminate between pathoge…
"What is life? Fifty years after physicist Erwin Schrodinger posed this question in his celebrated and inspiring book, the answer remains elusive. In The Way of the Cell, one of the world's most respected microbiologists draws on his wide knowledge of contemporary science to pro…