Lasers in cytology

This thesis investigates the application of a variety of optical techniques for the manipulation of single cells and their local micro-environment. The methodologies developed provide enhanced control over a single cell under study affording exquisite spatial and temporal contro…

In this thesis, femtosecond (fs) cellular transfection of Chinese Hamster Ovary (CHO) cells was performed using a tightly focused Gaussian beam. The beam focus was positioned on the cell membrane and three laser doses, each of 40 ms duration, were delivered allowing for the form…