The work described herein has explored solid-phase transduction of nucleic acid hybridization on platforms composed of paper substrates, and also glass surfaces within glass/polydimethylsiloxane microfluidic channels. Single-stranded probe oligonucleotides were conjugated to qua…
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The information age is upon us. We generate, process, store, display and transmit information in ever-increasing amounts. Sensing and measuring is one aspect of information generation that has become an increasingly important activity in the modern time. Sensors can be categoriz…
Integration and miniaturisation in electronics has undoubtedly revolutionised the modern world. In biotechnology, emerging lab-on-a-chip (LOC) methodologies promise all-integrated laboratory processes, to perform complete biochemical or medical synthesis and analysis encapsulate…
This thesis discusses the preliminary development of a microfluidic system for low concentration vapour analysis incorporating a novel analyte preconcentration method to extend vapour detection limits. The topicality of this subject is evidenced by the urgent requirement to dete…
Three projects are described in this thesis that combine microfabrication techniques with optical micromanipulation. The aim of these projects is to use expertise in microlithography and optical tweezing to create new tools for Lab-on-Chip devices. The first project looks at the…
Over recent years the delivery of nanosized drug particles has shown potential in improving bioavailability. Drug nanosizing is achieved by 'top-down' and by 'bottom-up' approaches. Owing to limitations associated with the top-down techniques, such as high energy input, electros…
The Life Marker Chip instrument, which has been selected to fly as part of the 2018 ExoMars rover mission payload, aims to detect up to 25 organic molecules in martian rocks and regolith, as markers of extant life, extinct life, meteoritic in-fall and spacecraft contamination. M…
“Lab on a Chip” technologies have revolutionized the field of bio-chemical analytics. The crucial role of optical techniques in this revolution resulted in the emergence of a field by itself, which is popularly termed as “optofluidics”. The miniaturization and integration of the…
The objective of this dissertation is to develop a mesoscopic multiscale particle based computational method, extend its applicability to state-of-the-art problems and simulate microfluidic processes at mesoscale, An in-house comprehensive computational package based on dissipat…
Genetic analysis is not widely used for disease diagnostics as it is costly and very labour/infrastructure intensive. We believe that by employing both microelectronic and microfabrication technologies, we are able to integrate multiple functionalities into a single, manufactura…
Dielectrophoresis, a nonlinear electrokinetic transport mechanism, becomes popular in many engineering and biomedical applications for manipulation, characterization and actuation of biomaterials, particles and biological cells. However, the underlying fundamental mechanism of d…
This experimental study investigates the feasibility of using a lens-less charge-coupled device (CCD) for the detection of organic and inorganic fluorophores. The study further investigates the feasibility of this device as a detection sensor in a hand-held Point-of-Care (POC) m…
Existing microfluidic devices transport fluids by a variety of physical means. Among them, pump-based and capillary-based fluid delivery systems are commonly adapted to move fluids. The pump-based systems intrinsically require sub systems consisting of mechanical and/or electric…
In this thesis work, designs were developed and implemented for separating plasma from a droplet of whole blood in an active and passive Lab-on-Chip (LOC) device. Nine filters were tested and compared to establish a process of elimination based on the filtration properties and p…