protein engineering

P450 BM3 is a natural fusion protein containing a heme catalytic domain fused to a CPR-like domain that binds two flavin cofactors (FAD and FMN). This allows this enzyme to have one of the highest catalytic rates of any P450. Such characteristics make P450 BM3 an attractive enzy…

Experiments facilitating the engineering of the PETNR active site to accommodate a range of non natural enone substrates with substituents localised on the α and β carbons of the unsaturated bond are described. In order to facilitate the high throughput purification of PETNR lib…

Metalloproteins are some of the best catalysts in Nature. The synergistic combination of transition metals and the appropriate protein scaffold creates catalysts that can accomplish even the most difficult chemical transformations with unmatched efficiency. Several approaches ha…