Single nucleotide polymorphisms (SNPs) are one of the forensic markers used to resolve the problem of DNA typing from degraded samples. It has been found in previous studies that when profiling heavily degraded forensic samples the small amplicon required for SNP analysis has an…
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Eukaryotic cells are highly compartmentalized, so that some steps of gene expression occur in the nucleus and others in the cytoplasm, i.e., transcription and pre-mRNA processing are spatially separated from translation which occurs in the cytoplasm. Ribosome subunits and severa…
Botulinum neurotoxin induces a potentially fatal paralytic condition in humans and various animal species collectively known as 'botulism'. It consequently poses a major problem to the food industry, due to the ability of its spores to survive in cooked foods. The incidence of w…
Experimental meningitis was induced in pigmented guinea pigs by subarachnoid inoculation of \(1 \times 10^9\) Escherichia coli K-12 or \(3 \times 10^7\) CFU Streptococcus pneumoniae serotype 2 D39 (NCTC 7466) or PLN-A, \(Δ\)NA1 or \(Δ\)HY1, defined isogenic derivatives of D39 de…
The central aim of this study was to characterise the expression of the candidate tumour suppressor gene, LV/-J (lentivirus integration-I) and its products. The LVI-J gene (WDR76) was discovered as a target for disruption by proviral insertional mutagenesis in a case of pre-B ce…
A sequence encoding a cathepsin B-like cysteine peptidase of the clan CA, family C1 was identified in the genome database of E. tenella. The sequence corresponded to a single copy gene, and did not carry any introns. The E. tenella enzyme, sharing 42% identity with the Toxoplasm…
The aim of this project was to investigate the possible role of the biofilm matrix as a barrier to drug diffusion in Candida biofilms and in mixed species fungal-bacterial biofilms. The penetration of antifungal agents through single- and mixed-species biofilms containing Candid…
mRNA was transcribed m vitro by the endogenous RNA transcriptase of calf rotavirus. This mRNA had a mean sedimentation coefficient of 12S, and hybridised to genomic double stranded RNA. Rotavirus mRNA appeared not to be polyadenylated as less than 1% of it was retained by oligo …
Sindbis virus an alphavirus, has a single stranded RNA genome with a molecular weight of 4.2 million (42S RNA). Inside infected cells two RNA species are found. One, 42S RNA, is assumed to code for the RNA polymerase(s) and is also destined to enter mature virus, the second, 26S…
A purification procedure was developed for Eschericia coli polynucleotide phosphorylase, and subsequently applied to polynucleotide phosphorylases from Thermus aquaticus and Bacillus stearothermophilus. Preliminary investigations of the catalytic properties of the thermostable p…
Differentiation in the budding photosynthetic bacterium Rhodopseudomonas palustris was studied both in its own right and in the context of other comparable bacteria; particularly R. acidophila and Rhodomicrobium vannielii. Together, these three Rhodospirillaceae present a gradie…
Human herpesvirus (HHV) –6A and HHV-6B are unique among herpesviruses in their ability to integrate into the telomeres of human chromosomes and be inherited in a Mendelian fashion. Based on current data, inherited chromosomally integrated HHV-6 (iciHHV-6) is present in 0.5-2% of…
Dental handpieces are used in a wide variety of dental treatment and oral surgery. During patient treatment handpieces becom contaminated with patient material. Due to the design and function of the dental handpieces, internal contamination of handpiece components frequently occ…
Traumatic injury to the spinal cord interrupts ascending and descending pathways leading to severe functional deficits of sensory motor and autonomic function which depend on the level and severity of the injury. There are currently no effective therapies for treating such injur…
Cystic Fibrosis (CF) is the commonest life limiting inherited disease illness in the western world. Over the last few decades there have been many advances in the diagnosis and management of this condition. Patients born with the disease now are living into their fourth decade, …
Obesity is one of the biggest public health problems today, leading to type II diabetes, coronary heart disease, hypertension, sterility and cancer (Hashmi et al., 2013). \(C.elegans\) has been used extensively in obesity research to dissect the underlying principles. The overar…
Introduction: Varying degrees of periodontal disease affect the majority of the population. Severe forms of periodontitis have a considerable impact on oral health and quality of life. Periodontitis results from imbalances in the oral microbiome and the host immune response. The…
Escherichia coli is a diverse bacterial species found largely as a harmless commensal in the gastrointestinal tract of warm-blooded mammals. However, an array of highly adapted E. coli pathotypes have evolved over time capable of causing a variety of niche specific diseases, bot…
Neurodegenerative conditions such as Multiple Sclerosis (MS) and spinal cord injury (SCI) affect hundreds of thousands of people each year worldwide, and numerous cell transplant-based therapeutic strategies are being investigated to aid in the repair and regeneration of the cen…