Thesiss

In this work it was demonstrated for the first time that Alkaline Phosphatase (ALP) enzymes spotted and dried onto a nitrocellulose membrane remained active. This was accomplished by passing a fixed volume of the BCIP/NBT substrate solution over the immobilized enzymes and measu…

The first two studies were designed to evaluate the effects of a bacterial phytase (Exp 1 and 2) and a fungal phytase (Exp. 2) on nutritional and physiological responses in weanling pigs. Pigs were weaned at 21d of age and housed individually. Treatments consisted of a negative …