Adipose-Derived Stem Cells: Methods and Protocols

by Bunnell, Bruce A. Gimble, Jeffrey M.

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Title Statements

Full title :Adipose-Derived Stem Cells : Methods and Protocols / edited by Jeffrey M. Gimble, Bruce A. Bunnell.

Summary

During the past decade, a wide range of scientific disciplines have adopted the use of adipose-derived stem/stromal cells (ASCs) as an important tool for research and discovery. In Adipose-Derived Stem Cells: Methods and Protocols, experts from the field, including members of the esteemed International Federation of Adipose Therapeutics and Science (IFATS), provide defined and established protocols in order to further codify the utilization of these powerful and accessible cells. With chapters organized around approaches spanning the discovery, pre-clinical, and clinical processes, much of the emphasis is placed on human ASC, while additional techniques involving small and large animal species are included. As a volume in the highly successful Methods in Molecular Biology"!series, the detailed contributions include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Adipose-Derived Stem Cells: Methods and Protocols serves as a vital reference text for experienced researchers as well as new students on the path to further exploring the incredible potential of ASCs

Edition Statements

Publication Date:2011.
Edition:1st ed. 2011

Publication Statements

Publisher(s):Humana, Humana Press, Imprint
Place of Publication:Totowa, NJ
Language:eng
Publication Date:2011.

Notes

Bibliographic Level Mode of Issuance: Monograph.

The Physical Object

Description:1 online resource (XVI, 474 pages)

Edition Identifiers

ISBN:1617379603, 9781617379604, 9781617379598, 161737959X
DOI:10.1007/978-1-61737-960-4.
oclc:on1164927566
LC Classification:QH573-671
Dewey Decimal:571.6

Isolation and Culture of Rhesus Adipose-Derived Stem Cells -- Isolation of Human Adipose-Derived Stem Cells from Lipoaspirates -- Isolation of Murine Adipose-Derived Stem Cells -- In vitro Adult Rat Adipose Tissue-Derived Stromal Cell Isolation and Differentiation -- In vitro Adult Canine Adipose Tissue-Derived Stromal Cell Growth Characteristics -- Adipogenic Differentiation of Adult Equine Mesenchymal Stromal Cells -- Isolation and Culture of Porcine Adipose Tissue-Derived Somatic Stem Cells -- Automated Isolation and Processing of Adipose-Derived Stem and Regenerative Cells -- Methods for the Purification and Characterization of Human Adipose-Derived Stem Cells -- Characterization of Human ASCs Using Flow Cytometry -- Evaluation of Cellular and Humoral Immune Responses to Allogeneic Adipose-Derived Stem/Stromal Cells -- Adipose-Derived Stromal Cells: Cytokine Expression and Immune Cell Contaminants -- Gel-Based and Gel-Free Proteomic Technologies -- Adipogenic Differentiation of Adipose-Derived Stem Cells -- 3D Culture Systems to Induce Chondrogenesis of Adipose-Derived Stem Cells -- Neural Differentiation of Human ASCs -- Osteogenic Differentiation Strategies for Adipose-Deriv…

Subjects

Adipose Tissue -- cytology Adipose tissues Cell Biology Cell culture Cell Culture Techniques Cells, Cultured Cellules -- Culture Cellules souches Cytologie Cytology Laboratory Manual Laboratory manuals Manuels de laboratoire Stem cells