Affinity Chromatography: Methods and protocols
The aim of this edition is to introduce the beginner to the basics of affinity chromatography and provide practical knowledge for the development of affinity separation protocols. Affinity Chromatography: Methods and Protocols, Third Edition guides readers through new state of the art protocols, molecular modelling, and the study of ligand-target interactions. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists o…
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- Full title :Affinity Chromatography : Methods and protocols / edited by Senta Reichelt.
Summary
The aim of this edition is to introduce the beginner to the basics of affinity chromatography and provide practical knowledge for the development of affinity separation protocols. Affinity Chromatography: Methods and Protocols, Third Edition guides readers through new state of the art protocols, molecular modelling, and the study of ligand-target interactions. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
Read More ↓Edition Statements
- Publication Date:2015.
- Edition:3. ed
Publication Statements
- Publisher(s):Humana Press
- Place of Publication:New York
- Language:eng
- Publication Date:2015.
The Physical Object
- Description:1 online resource (online resource (xiii, 341 s.)) : : illustrations
Edition Identifiers
- ISBN:9781493924479, 1493924478
- oclc:ocn942578194
- Dewey Decimal:543.8
Table of Contents
1 Affinity Chromatography: A Historical Perspective / David S. Hage and Ryan Matsuda -- PART I GENERAL MODES AND LIGANDS OF AFFINITY CHROMATOGRAPHY -- 2 Protein Purification by Aminosquarylium Cyanine Dye-Affinity Chromatography / Vânia C. Graça, Fani Sousa, Paulo F. Santos, and Paulo S. Almeida -- 3 One-Step Purification of Phosphinothricin Acetyltransferase Using Reactive Dye-Affinity Chromatography / Cunxi Wang, Thomas C. Lee, Kathleen S. Crowley, and Erin Bell -- 4 Antibody Purification from Human Plasma by Metal-Chelated Affinity Membranes / Handan Yavuz, Nilay Bereli, Fatma Yılmaz, Canan Armutcu, and Adil Denizli -- 5 Specific Recognition of Supercoiled Plasmid DNA by Affinity Chromatography Using a Synthetic Aromatic Ligand / Catarina Caramelo-Nunes and Cândida T. Tomaz -- 6 Cell Affinity Separations on Microfluidic Devices / Yan Gao, Wenjie Li, Ye Zhang, and Dimitri Pappas -- 7 Aptamer-Modified Magnetic Beads in Affinity Separation of Proteins / Guohong Zhu and Johanna-Gabriela Walter -- 8 The Strep-tag System for One-Step Affinity Purification of Proteins from Mammalian Cell Culture / Thomas Schmidt and Arne Skerra -- 9 Robotic High-Throughput Purification of Affinity-Tag…
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