Animal Cell Culture: Essential Methods

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This is a comprehensive research guide that describes both the key new techniques and more established methods. Every chapter discusses the merits and limitations of the various approaches and then provides selected tried-and-tested protocols, as well as a plethora of good practical advice, for immediate use at the bench. It presents the most accessible and comprehensive introduction available to the culture and experimental manipulation of animal cells. Detailed protocols for a wide variety of…

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Title Statements

Full title :Animal Cell Culture : Essential Methods.

Summary

Edition Statements

Publication Date:2011.

Publication Statements

Publisher(s):John Wiley & Sons
Place of Publication:Hoboken
Language:eng
Publication Date:2011.

The Physical Object

Description:1 online resource (709 pages)

Edition Identifiers

ISBN:9780470669822, 0470669829, 9780470666586, 0470666587, 9780470669815, 0470669810, 9780470975633, 0470975636
oclc:ocn742333230
LC Classification:QH585.2.A565 2011
Dewey Decimal:571.6381078

Cover; Title Page; Copyright; Dedication; Contributors; preface; Abbreviations; Protocols; 1 The Cell Culture Laboratory; 2: Sterilization; 3: Microscopy of Living Cells; 4: Basic Techniques and Media, the Maintenance of Cell Lines, and Safety; 5: Development and Optimization of Serum- and Protein-free Culture Media; 6: Cryopreservation and Banking of Cell Lines; 7: Primary Culture of Specific Cell Types and the Establishment of Cell Lines; 8: Cloning; 9: The Quality Control of Animal Cell Lines and the Prevention, Detection and Cure of Contamination; 10: Systems for Cell Culture Scale-up.

11: Good Laboratory Practice in the Cell Culture LaboratoryColor Plate; Index.