Title Statements
- Full title :Bacterial Pangenomics : Methods and Protocols / edited by Alessio Mengoni, Marco Galardini, Marco Fondi.
Summary
Bacterial genomics is a mature research interdisciplinary field, which is approached by ecologists, geneticists, bacteriologists, molecular biologists and evolutionary biologists working in medical, industrial and basic science. Thanks to the large diffusion of bacterial genome analysis, Bacterial Pangenomics: Methods and Protocols is able to provide the most recent methodologies about the study of bacterial pangenomes by covering the three major areas: the experimental methods for approaching bacterial pangenomics, the bioinformatic pipelines for analysis and annotation of sequence data, and finally the methods for inferring functional and evolutionary features from the pangenome. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
Read More ↓Edition Statements
- Publication Date:2015.
Publication Statements
- Publisher(s):Humana Press
- Place of Publication:New York
- Language:eng
- Publication Date:2015.
The Physical Object
- Description:1 online resource (online resource (x, 298 s.)) : : illustrations
Edition Identifiers
- ISBN:9781493917204, 149391720X
- oclc:ocn941370814
- Dewey Decimal:572.86293
Table of Contents
1 Pulsed Field Gel Electrophoresis and Genome Size Estimates / Rosa Alduina and Annalisa Pisciotta -- 2 Comparative Analyses of Extrachromosomal Bacterial Replicons, Identification of Chromids, and Experimental Evaluation of Their Indispensability / Lukasz Dziewit and Dariusz Bartosik -- 3 Choice of Next-Generation Sequencing Pipelines / F. Del Chierico, M. Ancora, M. Marcacci, C. Cammà, L. Putignani, and Salvatore Conti -- 4 The Pyrosequencing Protocol for Bacterial Genomes / Ermanno Rizzi -- 5 Bacterial Metabarcoding by 16S rRNA Gene Ion Torrent Amplicon Sequencing / Elio Fantini, Giulio Gianese, Giovanni Giuliano, and Alessia Fiore -- 6 The Illumina-Solexa Sequencing Protocol for Bacterial Genomes / Zhenfei Hu, Lei Cheng, and Hai Wang -- 7 High-Throughput Phenomics / Carlo Viti, Francesca Decorosi, Emmanuela Marchi, Marco Galardini, and Luciana Giovannetti -- 8 Comparative Analysis of Gene Expression: Uncovering Expression Conservation and Divergence Between Salmonella enterica Serovar Typhimurium Strains LT2 and 14028S / Paolo Sonego, Pieter Meysman, Marco Moretto, Roberto Viola, Kris Laukens, Duccio Cavalieri, and Kristof Engelen -- 9 Raw Sequence Data and Quality Control / G…
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