Beta-Arrestins: Methods and Protocols

by Laporte, Stéphane A. Scott, Mark G. H.

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Title Statements

Full title :Beta-Arrestins : Methods and Protocols / edited by Mark G. H. Scott, Stéphane A. Laporte.

Summary

This volume looks at various approaches to study the pleiotropic roles of b-arrestins (b-arrs) in the control of signal transduction, and the resulting cellular and in vivo consequences that arise. The chapters in this book cover diverse topics around b-arrs such as their established roles in GPCR regulation and trafficking; regulatory scaffolding functions of b-arrs in MAPK signaling, cAMP hydrolysis and cytoskeletal dynamics; proteomic analysis of the b-arr interactome; mathematical modelling of b-arr signaling networks; functional selectivity involving biased ligands; nucleocytoplasmic trafficking and primary cilia-associated functions of b-arrs; conformational plasticity of b-arrs; and the roles of b-arrs in allergic inflammation, Type 2 Diabetes, Parkinson's disease, and cancer. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, Beta-Arrestins: Methods and Protocols is a valuable resource for researchers interested in learning more about the function and regulation of b-arrestins.

Edition Statements

Publication Date:2019.

Publication Statements

Publisher(s):Humana Press, Imprint, Springer New York
Place of Publication:New York, NY
Language:eng
Publication Date:2019.

The Physical Object

Description:1 online resource (XIV, 409 pages 79 illustrations, 60 illustrations in color.) : : online resource

Edition Identifiers

ISBN:9781493991587, 1493991582
DOI:10.1007/978-1-4939-9158-7
oclc:on1098211743
LC Classification:QD431-431.7
Dewey Decimal:572.6

A Brief History of the b-Arrestins -- b-Arrestins: Multitask Scaffolds Orchestrating the Where and When In Cell Signaling -- Methods to Monitor the Trafficking of [beta]-Arrestin/G Protein-Coupled Receptor Complexes using Enhanced Bystander BRET -- Methods to Investigate b-Arrestin-Mediated Regulation of GPCR Function in Human Airway Smooth Muscle -- Measuring Recruitment of b-Arrestin to G Protein-Coupled Heterodimers using Bioluminescence Resonance Energy Transfer -- Detection of [beta]-Arrestin-Mediated G Protein-Coupled Receptor Ubiquitination using BRET -- Using In Vitro Pull-Down and In Cell Overexpression Assays to Study Protein Interactions with Arrestin -- Methods to Investigate Arrestins in Complex with Phosphodiesterases -- Methods to Characterize Protein Interactions with [beta]-arrestin in cellulo -- Methods to Investigate the [beta]-Arrestin-Mediated control of ARF6 Activation to Regulate Trafficking and Actin Cytoskeleton Remodeling -- Methods to Investigate the Roles of b-Arrestin-Dependent RalGDS Activation in GPCR-Stimulated Membrane Blebbing -- Methods to Determine Interaction Interfaces between b-Arrestins and Their Protein Partners -- Workflow Description to D…

Subjects

beta-Arrestins biochemistry Biochimie Cell Biology Cell receptors Cellular signal transduction Cytologie Cytology Récepteurs cellulaires Receptors, Cell Surface Receptors, G-Protein-Coupled Signal Transduction Transduction du signal cellulaire