Chromatin: methods and protocols

by Horsfield, Julia Marsman, Judith

Type Book

Rate 0.0 /5

Details

Files

Reviews

Download

Title Statements

Full title :Chromatin : methods and protocols / edited by Julia Horsfield, Judith Marsman.

Summary

This volume provides cutting-edge techniques to further the study chromatin biology. Chapters include both novel and well-established methods for the analysis of DNA-associated proteins, DNA methylation, three-dimensional chromatin interactions, deep sequencing-based tools, and data analysis pipelines. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, provides details of the necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and describes step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Chromatin: Methods and Protocols aims to further the understanding of how modified DNA and associated proteins affect the transcriptional output of the genome. Chapter Genome-wide mapping and microscopy visualization of protein-DNA interactions by pA-DamID [Chapter 12] is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Edition Statements

Publication Date:©2022

Publication Statements

Publisher(s):Humana Press
Place of Publication:New York, NY
Language:eng
Publication Date:©2022

The Physical Object

Description:1 online resource (1 PDF file (xi, 379 pages)) : : illustrations (chiefly color).

Edition Identifiers

ISBN:9781071621400, 1071621408
DOI:10.1007/978-1-0716-2140-0
oclc:on1296378512
LC Classification:QH599
Dewey Decimal:572.8/7

Generating sequencing-based DNA methylation maps from low DNA input samples -- Data analysis of DNA methylation Epigenome-Wide Association Studies (EWAS); A guide to the principles of best practice -- Next-generation bisulfite sequencing for targeted DNA methylation analysis -- Editing of DNA Methylation Patterns Using CRISPR-Based Tools -- Nanopore sequencing and data analysis for base-resolution genome-wide 5-methylcytosine profiling -- Chromatin immunoprecipitation sequencing (ChIP-seq) protocol for small amounts of frozen biobanked cardiac tissue -- A robust protocol for investigating the cohesin complex by ChIP-sequencing -- Epi-Decoder: decoding the local proteome of a genomic locus by massive parallel chromatin immunoprecipitation combined with DNA-barcode sequencing -- A protocol for studying transcription factor dynamics using fast Single-Particle Tracking and Spot-On model-based analysis -- Characterization of mammalian regulatory complexes at single locus resolution using TINC -- Profiling protein-DNA interactions cell-type-specifically with Targeted DamID -- Genome-wide mapping and microscopy visualization of protein-DNA interactions by pA-DamID -- The dCypher approach…