Clinical proteomics: methods and protocols

by Corrales, Fernando J. Marcilla, Miguel Paradela, Alberto

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Title Statements

Full title :Clinical proteomics : methods and protocols / edited by Fernando J. Corrales, Alberto Paradela, Miguel Marcilla.

Summary

This detailed volume explores techniques in clinical proteomics, an emerging discipline aimed at deciphering the molecular mechanisms underlying the progression of diseases and at identifying new biomarkers and therapeutic targets to expand the physicians' toolbox for precision medicine-based patient care. From sample processing to multi-omics approaches, the book provides straight-forward protocols on a wide array of vital areas of study. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Clinical Proteomics: Methods and Protocols serves as an ideal guide for researchers working to expand upon the knowledge base needed to push forward toward a more personalized version of medicine.

Edition Statements

Publication Date:2022

Publication Statements

Publisher(s):Humana Press
Place of Publication:New York, NY
Language:eng
Publication Date:2022

The Physical Object

Description:xiii, 264 pages : : illustrations (some color) ; : 26 cm.

Edition Identifiers

ISBN:9781071619353, 1071619357
oclc:on1260132268
LC Classification:QP551
Dewey Decimal:572.6

Bile Processing Protocol for Improved Proteomic Analysis -- MS-Based Extracellular Vesicles (EVs) Analysis: An Application to Helminth-Secreted EVs -- Proteomic Profiling of Cerebrospinal Fluid by 16-Plex TMT-Based Mass Spectrometry -- Data-Independent Acquisition Mass Spectrometry-Based Deep Proteome Analysis for Hydrophobic Proteins from Dried Blood Spots Enriched by Sodium Carbonate Precipitation -- Sample Processing for Metaproteomic Analysis of Human Gut Microbiota -- Mass Spectrometry-Based Analytical Strategy for Single Cell Proteomics -- Lysine Acetylation Stoichiometry Analysis at the Proteome Level -- Implementation of Clinical Phosphoproteomics and Proteomics for Personalized Medicine -- pH/Acetonitrile-Gradient-Reversed-Phase Fractionation of Enriched Hyper-Citrullinated Library in Combination with LC-MS/MS Analysis for Confident Identification of Citrullinated Peptides -- Purification of HLA Immunopeptidomes from Human Thymus -- Generation of HLA Allele-Specific Spectral Libraries to Identify and Quantify Immunopeptidomes by SWATH/DIA-MS -- Immunopeptidomic Analysis of the Phosphopeptidome Displayed by Hla Class I Molecules -- Development of a Standardized MRM Method …